Development of a rapid and sensitive magnetic chemiluminescent enzyme immunoassay for detection of luteinizing hormone in human serum.
Development of a rapid and sensitive magnetic chemiluminescent enzyme immunoassay for detection of luteinizing hormone in human serum.
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DOI：
10.1016/j.clinbiochem.2009.06.021
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发表时间：
2009-09
影响因子：
2.8
通讯作者：
Qin Xiao;Haifang Li;Guo-mao Hu;Hongrui Wang;Zhen-jia Li;Jin‐Ming Lin
中科院分区：
文献类型：
--
作者：
Qin Xiao;Haifang Li;Guo-mao Hu;Hongrui Wang;Zhen-jia Li;Jin‐Ming Lin
目的建立基于磁性微粒(MPS)的快速、灵敏的化学发光酶免疫分析法(MPS-CLEIA)测定人血清中黄体生成素(LH)的方法。以辣根过氧化物酶-鲁米诺-过氧化氢化学发光体系为检测体系，方法的检出限为0.2 mIU/m L，线性范围为0.5-200 m IU/m L。该方法无交叉反应，批内和批间变异系数均小于10%。加标回收率为101.6%~104.1%。该方法已成功应用于人血清中黄体生成素的检测，并与放射免疫法(RIA)进行了良好的相关性。结论MPS-CLEIA较RIA具有明显的优势，有助于临床高通量筛查和自动化操作系统的发展。
OBJECTIVESA rapid and sensitive magnetic particles (MPs)-based chemiluminescence enzyme immunoassay (MPs-CLEIA) for determination of luteinizing hormone (LH) in human serum was developed and validated.DESIGN AND METHODSThe method was developed based on a sandwiched immunoreaction format, in which MPs served as both the solid phase and separator. The horseradish peroxidase(HRP)–luminol–H2O2chemiluminescent system was chosen as the detection system.RESULTSThis method had a detection limit of 0.2 mIU/mL and a linear range of 0.5–200 mIU/mL. The assay showed little cross-reactivity, and the intra- and inter-assay coefficients of variation were less than 10%. The recoveries were from 101.6% to 104.1%. The method has been successfully applied to detection of LH in human serum and showed good correlation compared with radioimmunoassay (RIA).CONCLUSIONThe MPs-CLEIA provided apparent advantages over RIA, and facilitated the development of high-throughput screening and automated operation systems in clinical diagnosis.