Prevalence of genes encoding exfoliative toxins among Staphylococcus hyicus isolated in Russia and Germany
Prevalence of genes encoding exfoliative toxins among Staphylococcus hyicus isolated in Russia and Germany
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DOI：
10.1111/j.1439-0450.2006.00988.x
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发表时间：
2006-11-01
期刊：
影响因子：
--
通讯作者：
Gintsburg, A. L.
中科院分区：
文献类型：
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作者：
Kanbar, T.;Voytenko, A. V.;Gintsburg, A. L.
在本研究中，先前在俄罗斯（n = 23）和德国（n = 17）分离的猪葡萄球菌的特征进行了调查的流行剥脱毒素编码基因exhA，exhB，exhC和exhD的多重PCR检测exhD阳性菌株中的S。对俄罗斯猪渗出性表皮炎分离株和德国猪渗出性表皮炎分离株分别进行exhC和exhD检测。毒素基因阴性菌株通常分离自表面健康的猪、其他动物以及分离到的沙门氏菌与猪链球菌的关系密切的标本。hyicus和临床症状仍不清楚。对所选exhC和exhD阳性菌株的毒素基因进行部分测序，并将测序结果与exhC和exhD参考菌株的序列进行比较，结果显示几乎完全相同。本研究的结果与Andresen和Ahrens（J.Appl.Microbiol.，96，2004，1265）和Andresen（J.Vet.Rec.，157，2005，376），提出的多重PCR可用于调查S. hyicus的产毒潜力，并有毒素基因的存在之间的关联，在S。渗出性表皮炎的Hyicus菌株。然而，与S.在德国分离的猪属菌株，其先前由Andresen（J.Vet.Rec.，157，2005，376），exhD似乎在S.来自俄罗斯的hyicus菌株。
In the present study, previously characterized Staphylococcus hyicus isolated in Russia (n = 23) and Germany (n = 17) were investigated for the prevalence of the exfoliative toxin encoding genes exhA, exhB, exhC and exhD by multiplex PCR resulting in the detection of exhD positive strains among the S. hyicus isolated from pigs with exudative epidermitis in Russia and the detection of exhC and exhD for one and two strains isolated from exudative epidermitis in Germany respectively. The toxin gene negative strains were generally isolated from apparently healthy pigs, from other animals and from specimens where the relation between the isolation of S. hyicus and the clinical symptoms remained unclear. Partial sequencing of the toxin genes of selected exhC and exhD positive strains and comparing the sequencing results with sequences of exhC and exhD reference strains revealed an almost complete identity. The results of the present study were in agreement with the findings of Andresen and Ahrens (J. Appl. Microbiol., 96, 2004, 1265) and Andresen (J. Vet. Rec., 157, 2005, 376) that the presented multiplex PCR could be used to investigate S. hyicus for toxinogenic potential and that there is an association between the presence of toxin genes in S. hyicus strains from exudative epidermitis. However, comparable with the S. hyicus strains isolated in Germany which were investigated previously by Andresen (J. Vet. Rec., 157, 2005, 376), exhD seems to predominate in S. hyicus strains from Russia.