Evaluation of the action of Tween 20 non-ionic surfactant during enzymatic hydrolysis of lignocellulose: Pretreatment, hydrolysis conditions and lignin structure
Evaluation of the action of Tween 20 non-ionic surfactant during enzymatic hydrolysis of lignocellulose: Pretreatment, hydrolysis conditions and lignin structure
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DOI：
10.1016/j.biortech.2018.08.119
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发表时间：
2018-12-01
影响因子：
11.4
通讯作者：
Zhao, Xuebing
中科院分区：
文献类型：
--
作者：
Chen, Yu-An;Zhou, Yan;Zhao, Xuebing
研究了预处理工艺、水解条件和木质素结构特征对表面活性剂（Tween 20）促进预处理麦草酶解的影响，并进一步解释了这些因素与纤维素酶在木质素上非生产性吸附的关系。吐温20似乎在更苛刻的条件下更大地提高纤维素转化率。表面活性剂表现出更显着的改善比氧化预处理基板酸预处理基板。高浓缩度的木质素和酚羟基对纤维素酶有较强的吸附能力，而吐温20能很好地阻断木质素-纤维素的相互作用，恢复纤维素的水解能力。预处理改变了木质素结构，导致表面性质的变化，从而进一步影响木质素-纤维素酶的相互作用。吐温20的加入可以改变木质素的表面性质，改变其疏水性、氢键能力和表面电荷，从而减少蛋白质的非生产性吸附。
The aim of this work was to study the effects of pretreatment process, hydrolysis condition and structural features of lignin on the improving action of surfactants (Tween 20) for enzymatic hydrolysis of pretreated wheat straw, and further to interpret the relation of these factors with the non-productive adsorption of cellulases on lignin. Tween 20 seemed to be more greatly improve cellulose conversion under harsher conditions. The surfactant showed more significant improvement for acid-pretreated substrates than oxidative-pretreated substrates. Highly-condensed lignin and phenolic hydroxyl groups showed much stronger adsorption ability to cellulases, while Tween 20 could well block the lignin-cellulase interactions recovering cellulose hydrolyzability. It was proposed that pretreatments altered lignin structures, resulting in the change of surface properties thus further impacting the lignin-cellulase interactions. Addition of Tween 20 could modify lignin surface properties to change its hydrophobicity, hydrogen bonding ability and surface charges, thus reducing the nonproductive adsorption of proteins.