Spatio-temporally differential expression of genes for three members of fatty acid binding proteins in developing and mature rat brains
Spatio-temporally differential expression of genes for three members of fatty acid binding proteins in developing and mature rat brains
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DOI：
10.1016/s0891-0618(96)00192-5
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发表时间：
1996-12-01
影响因子：
2.8
通讯作者：
Kondo, H
中科院分区：
文献类型：
--
作者：
Owada, Y;Yoshimoto, T;Kondo, H
用Northern杂交和原位杂交技术研究了大鼠脑内三种胞质脂肪酸结合蛋白(FABP)基因表达的时间变化。心脏(H)-FABP的表达在出生后开始明显，并逐渐增加，并局限于灰质，提示H-FABP在出生后脑内的表达是神经元特异性的。脑(B)-FABP在脑室生发区表达较强，在小脑外颗粒细胞层无表达，提示其主要表达于神经胶质系细胞。B-FABP基因在围产期的灰质和白质有短暂表达，成人期仅在嗅神经纤维层有胶质细胞表达。另一方面，皮肤型(S)-FABP在脑室生发区和小脑外颗粒细胞层均有明显表达，提示在神经元系细胞中有表达。S-FABP在出生前灰质有明显表达，S-FABP在出生后早期在神经胶质细胞中有表达，之后在成年脑中表达下降，但仍维持在弱水平。这三种FABP在不同发育阶段的神经元和神经胶质细胞中具有不同的功能。版权所有(C)1996 Elsevier Science B.V.
The chronological changes in the gene expression for three species of cytosolic fatty acid-binding proteins (FABPs) in the rat brain were examined by Northern and in situ hybridization analyses. The expression for heart(H)-FABP became evident after birth, with a gradual increase and confined to the gray matter, suggesting that the expression of H-FABP mRNA is neuron-specific in postnatal brain. The expression for brain(B)-FABP was very intense in the ventricular germinal zone, without expression in the cerebellar external granule cell layer, suggesting the dominant expression in the cells of glial lineage. B-FABP mRNA was transiently expressed in perinatal gray as well as white matter and the expression in glial cells persists only in the olfactory nerve fiber layer at the adult stage. On the other hand, the expression for skin type(S)-FABP was evident in the both ventricular germinal zone and cerebellar external granule cell layer, suggesting the expression in cells of neuronal lineage. The expression for S-FABP was evident in the prenatal gray matter and S-FABP mRNA was expressed in glial cells at early postnatal stage, whereafter the expression decreased to, but remained at weak levels in the adult brain. Discrete functions of the three FABPs were suggested in neurons and glia differentially at various developmental stages. Copyright (C) 1996 Elsevier Science B.V.