Expression of the facilitated glucose transporters (GLUT1 and GLUT3) by a choriocarcinoma cell line (JAr) and cytotrophoblast cells in culture
Expression of the facilitated glucose transporters (GLUT1 and GLUT3) by a choriocarcinoma cell line (JAr) and cytotrophoblast cells in culture
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DOI：
10.1016/s0143-4004(97)80068-9
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发表时间：
1997-05-01
期刊：
影响因子：
3.8
通讯作者：
Sibley, CP
中科院分区：
文献类型：
--
作者：
Clarson, LH;Glazier, JD;Sibley, CP
研究人胎盘滋养层细胞中GLUT 1和GLUT 3 mRNA和蛋白的表达。分裂绒毛膜癌衍生的细胞系（JAr）进行了比较，分化细胞滋养层细胞，分离自人类足月胎盘，18（单核）和66小时（多核）的文化。还研究了用8-溴腺苷处理的JAr细胞，其抑制生长并诱导分化。GLUT 1 mRNA和蛋白表达在四组细胞中相似。然而，GLUT 3 mRNA的表达显着更高（六至七倍），在控制和8-溴腺苷处理的JAr细胞相比，细胞滋养层细胞，也显着更高，在未处理与处理的JAr细胞。Western blotting结果显示，GLUT 3蛋白在两组滋养层细胞中均未检测到，但在两组JAr细胞中均表达丰富。用8-溴腺苷处理的JAr细胞中的GLUT 3蛋白也显著低于未处理的JAr细胞，与mRNA数据一致。我们得出结论，GLUT 1的表达是不受滋养层细胞的生长或分化，而GLUT 3的表达与分裂细胞。我们认为，在胎盘中，GLUT 3可能参与维持分裂滋养层细胞的代谢需求，而不是直接参与葡萄糖转运到胎儿。(C)小行星1997 W. B。Saunders Company Ltd
The expression of GLUT1 and GLUT3 mRNA and protein in human placental trophoblast-derived cells was investigated. A dividing choriocarcinoma derived cell line (JAr) was compared to differentiating cytotrophoblast cells, isolated from human term placenta, following 18 (mononucleate) and 66 h (multinucleate) in culture. JAr cells treated with 8-bromoadenosine, which inhibits growth and induces differentiation, were also studied. GLUT1 mRNA and protein expression were similar in the four groups of cells. However, GLUT3 mRNA expression was significantly higher (six- to sevenfold) in both control and 8-bromoadenosine-treated JAr cells compared to cytotrophoblast cells and was also significantly higher in untreated versus treated JAr cells. Western blotting showed that GLUT3 protein was undetectable in either cytotrophoblast cell groups, but was abundant in both groups of JAr cells. GLUT3 protein in JAr cells treated with 8-bromoadenosine was also significantly lower than in untreated JAr cells, in agreement with the mRNA data. We conclude that GLUT1 expression is unaffected by either growth or differentiation of trophoblast cells whereas GLUT3 expression is associated with dividing cells. We propose that in the placenta, GLUT3 may be involved in maintaining metabolic requirements of dividing trophoblast cells, rather than having a direct role in transport of glucose to the fetus. (C) 1997 W. B. Saunders Company Ltd.