Urinary metabonomics of stomach cancer assessed by rapid resolution liquid chromatography/time‐of‐flight mass spectrometry
Urinary metabonomics of stomach cancer assessed by rapid resolution liquid chromatography/time‐of‐flight mass spectrometry
复制标题
通过快速分辨液相色谱/飞行时间质谱评估胃癌的尿液代谢组学
DOI：
10.3760/cma.j.issn.0366-6999.20111240
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发表时间：
2013
影响因子：
6.1
通讯作者：
Shu
中科院分区：
文献类型：
--
作者：
Yan Zhang;Hui Ren;Yang Jiang;Yufei Gao;Shu
胃癌是世界上最常见的恶性肿瘤之一。开发一种基于尿液的检测方法是有益的，这样可以对未确诊的胃癌患者进行筛查，并在早期发现他们的癌症。方法采用超高效液相色谱-四倍飞行时间质谱联用的尿液代谢组学方法对胃癌患者和健康对照者的尿液进行分析。结果采用上述方法对患者和健康对照组进行了明确的区分。鉴定出一些显著变化的代谢物。结论应用代谢组学方法可有效检测胃癌患者尿液代谢物的明显变化，具有检测癌症的能力；因此，它可能是早期诊断的一个有价值的工具。此外，在本研究中检测和鉴定改变的代谢物可能有助于阐明可能参与胃癌的机制。
Background Stomach cancer is among the most commonly occurring malignancies worldwide. It would be beneficial to develop a urine‐based assay whereby patients with undiagnosed stomach cancer could be screened and their cancer detected in the earliest stages. Methods A urinary metabonomics method based on ultra‐performance liquid chromatography combined with quadruple time‐of‐flight mass spectrometry was used to analyze urine samples from patients with stomach cancer and healthy controls. Results Statistical analysis revealed a clear separation of patients and healthy controls using the aforementioned methodology. Some significantly changed metabolites were identified. Conclusions Use of the metabonomics method in patients with stomach cancer could effectively detect distinct changes in urinary metabolites and had the capacity to detect cancer; therefore, it may be a valuable tool in earlier diagnosis. Furthermore, the detection and identification of altered metabolites in the current study may help elucidate possible mechanisms involved in stomach cancer.