SYNTHESIS OF GLYCOPROTEINS IN HUMAN-MELANOMA CELLS INFECTED WITH VARICELLA-ZOSTER VIRUS
SYNTHESIS OF GLYCOPROTEINS IN HUMAN-MELANOMA CELLS INFECTED WITH VARICELLA-ZOSTER VIRUS
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DOI：
10.1016/0042-6822(80)90478-x
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发表时间：
1980-01-01
期刊：
影响因子：
3.7
通讯作者：
GROSE, C
中科院分区：
文献类型：
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作者：
GROSE, C
研究了水痘带状疱疹病毒（VZV）感染人黑色素瘤细胞前后糖蛋白的合成。糖基化的多肽用糖部分的氚化前体标记，并通过聚丙烯酰胺凝胶电泳进行分析。当未感染和感染的细胞裂解物的电泳图谱进行比较时，在病毒感染的后期，宿主细胞糖蛋白合成明显减少。检测到至少3种感染细胞特异性（ICS）糖基化多肽。在用人和兔VZV抗血清免疫沉淀[3H]葡糖胺标记的VZV细胞提取物后，鉴定了5种ICS糖蛋白，并根据它们的表观MW（X）将其命名为gp 45、gp 62、gp 88、gp 98和gp 118。103）。在用[3H]岩藻糖标记的感染细胞提取物的免疫沉淀后，5种多肽中的4种（但不是gp 88）可见。ICS糖肽的MW对应于[35S]甲硫氨酸标记的多肽中检测到的病毒富集的馏分通过离心超声破碎的感染细胞的组合密度-粘度梯度。一个突出的ICS高MW（约150，000）非糖基化多肽。
The synthesis of glycoproteins was investigated in cultured human melanoma cells before and after infection with varicella-zoster virus (VZV). The glycosylated polypeptides were labeled with tritiated precursors of the sugar moiety and were analyzed by polyacrylamide gel electrophoresis. When the electrophoretic profiles of uninfected and infected cell lysates were compared, a marked reduction in host cell glycoprotein synthesis was evident during the late phase of viral infection. At least 3 infected-cell specific (ICS) glycosylated polypeptides were detected. After immune precipitation of [3H]glucosamine-labeled VZV cell extracts with human and rabbit VZV antisera, 5 ICS glycoproteins were identified and designated gp 45, gp 62, gp 88, gp 98 and gp 118 according to their apparent MW (.times. 103). Four of the 5 polypeptides (but not gp 88) were visualized after immune precipitation of infected cell extracts labeled with [3H]fucose. The ICS glycopeptides corresponded in MW to [35S]methionine-labeled polypeptides detected in a virus-enriched fraction obtained by centrifugation of sonically disrupted infected cells in a combination density-viscosity gradient. A prominent ICS high-MW (.apprx. 150,000) nonglycosylated polypeptide was identified also in the latter fraction.