Intracellular transport of basement membrane-type heparan sulphate proteoglycan in adenoid cystic carcinoma cells of salivary gland origin: an immunoelectron microscopic study
Intracellular transport of basement membrane-type heparan sulphate proteoglycan in adenoid cystic carcinoma cells of salivary gland origin: an immunoelectron microscopic study
复制标题
DOI：
10.1007/s004280050214
复制
发表时间：
1998-07-01
期刊：
影响因子：
3.5
通讯作者：
Saku, T
中科院分区：
文献类型：
--
作者：
Irie, T;Cheng, J;Saku, T
ACC3是一种源自唾液腺的人腺样囊性癌细胞系，在体外能够合成并分泌大量基底膜分子。为了确定这些分子的超微结构分泌途径，我们对培养7天的ACC3中的硫酸乙酰肝素蛋白聚糖（HSPG）进行了免疫定位。在培养早期，免疫标记的主要区域是粗面内质网（rER）和小分泌囊泡。在接种后的第3到4天，注意到HSPG定位在核周部分扩张的空间、粗面内质网和高尔基池，以及溶酶体或那些与多泡体和内体融合的结构中。在第5天及以后，几乎每个高尔基体都显示出池的明显扩张，并且HSPG在这些扩张的空间中被免疫定位。在培养后期，同时标记有HSPG和组织蛋白酶D的自噬泡或次级溶酶体在细胞质中积累。从第1天起就清楚地显示出细胞间隙中有HSPG沉积，并且在培养过程中增加。结果表明，ACC3细胞的HSPG周转周期增强：不仅是其生物合成，还包括内源性或外源性HSPG的降解。这种细胞内事件可能反映在腺样囊性癌的特征性组织学和生物学行为中。
ACC3, a human adenoid cystic carcinoma cell system of salivary gland origin, is able to synthesize and secrete a large amount of basement membrane molecules in vitro. To define the ultrastructural secreting pathway of these molecules, we immunolocalized heparan sulphate proteoglycan (HSPG) in ACC3 for 7 days of culture. In the early stage of culture, the main compartments immunolabelled were rough endoplasmic reticulum (rER) and small secretory vesicles. From days 3 to 4 after plating, it was noticed that HSPG was localized in partially dilated spaces of the perinuclear, rER and Golgi cisternae and in lysosomes or those fused with multivesicular bodies and endosomes. On and after day 5, almost every Golgi apparatus showed marked dilatation of the cisternae and HSPG was immunolocalized in these dilated spaces. In the later stage of culture, autophagic vacuoles or secondary lysosomes, which were simultaneously labelled for HSPG and cathepsin D, were accumulated in the cytoplasm. HSPG deposition in the intercellular space was clearly demonstrated from day 1 and increased during the culture. The results indicate that ACC3 cells have an enhanced turnover cycle for HSPG: not only its biosynthesis but also degradation of both endogenous or exogenous HSPG. Such intracellular events may be reflected in the characteristic histology and biological behaviour of adenoid cystic carcinomas.