Genetically driven assembly of nanorings based on the M13 virus
Genetically driven assembly of nanorings based on the M13 virus
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DOI：
10.1021/nl0347536
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发表时间：
2004-01-01
期刊：
影响因子：
10.8
通讯作者：
Belcher, AM
中科院分区：
文献类型：
--
作者：
Nam, KT;Peelle, BR;Belcher, AM
通过编码结合肽的两个遗传修饰和异双功能接头分子的合成，构建了来自M13病毒的一维环状结构。双功能病毒在病毒的相对末端展示抗链霉亲和素肽和六组氨酸肽，作为pIII和pIX融合体。化学计量添加的链霉亲和素-NiNTA接头分子导致可逆形成的病毒为基础的纳米环与周长对应的长度的可包装的DNA。这些基于病毒的环结构将被进一步设计成使无机材料成核，并使用三功能化病毒形成金属、磁性或半导体纳米环。
One-dimensional ring structures from M13 viruses were constructed by two genetic modifications encoding binding peptides and synthesis of a heterobifunctional linker molecule. The bifunctional viruses displayed an anti-streptavidin peptide and hexahistidine peptide at opposite ends of the virus as pIII and pIX fusions. Stoichiometric addition of the streptavidin-NiNTA linker molecule led to the reversible formation of virus-based nanorings with circumferences corresponding to lengths of the packagable DNAs. These virus-based ring structures will be further engineered to nucleate inorganic materials and form metallic, magnetic, or semiconductor nanorings using trifunctionalized viruses.