LFA-1 expression in a series of colorectal adenocarcinomas.
LFA-1 expression in a series of colorectal adenocarcinomas.
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DOI：
10.1007/s12029-011-9341-6
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发表时间：
2012-09-01
影响因子：
1.6
通讯作者：
Lazaris, Andreas C
中科院分区：
文献类型：
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作者：
Papas, Maria G;Karatzas, Pantelis S;Lazaris, Andreas C
前言：LFA-1是一种黏附分子，属于β2整合素家族。LFA-1在肝脏自然杀伤细胞中的过度表达与结直肠癌中肿瘤细胞的凋亡增加有关；此外，在结直肠癌中的研究已将LFA-1在肿瘤细胞中的过度表达与通过与内皮细胞黏附的血管侵入联系起来，从而暗示LFA-1可能在转移的发生中起作用。对福尔马林固定的石蜡包埋组织切片进行标准的三步免疫组织化学分析。采用抗CD11a的IgG2a单抗。根据性别、年龄、肿瘤部位、大小、分级、Dukes分期、壁层侵犯、有无转移或远处转移等临床病理因素进行分析。结果：LFA-1在51例原发肿瘤和6/33例转移淋巴结中表达。Dukes D组4例，仅1例LFA-1(+)。原发灶LFA-1的表达与无转移性疾病和Dukes B分期相关。结论：原发灶为LFA-1(+)的结直肠癌结直肠癌LFA-1表达与其微环境的适应优势有关，提示结直肠癌中LFA-1在结直肠癌结直肠癌的形成中起一定作用。
INTRODUCTION: LFA-1 is an adhesion molecule which belongs to the beta2-integrin family. Overexpression of LFA-1 in hepatic natural killer cells has been associated with increased apoptosis of neoplastic cells in colorectal cancer (CRC); moreover, studies in CRC have linked LFA-1 overexpression in neoplastic cells with vascular intrusion through adhesion to endothelial cells, thus implying a possible role in creation of metastases.AIMS AND METHODS: We studied the expression of LFA-1 in a series of 82 patients with CRC. A standard three-step immunohistochemical analysis was performed on formalin-fixed, paraffin-embedded tissue sections. An IgG2a anti-CD11a monoclonal antibody was used. Cases were characterized according to clinicopathological variables including sex, age, tumor localization, size, grade, Dukes stage, wall invasion, and presence of metastatic lymph nodes (mLNs) or distal metastases.RESULTS: LFA-1 was expressed at the primary tumor site in 51 cases and 6/33 cases with metastatic lymphnodes. In Dukes D cases (n=4), only one case was LFA-1(+). LFA-1 expression at the primary tumor site was associated with the absence of metastatic disease and with Dukes B stage. However, in those cases with LFA-1 expression in cancer cells in mLNs, this was associated with its expression at the primary tumor site.CONCLUSION: The positive association of LFA-1 expression in mLNs when the primary tumor site is also LFA-1(+) could imply an adaptation advantage of this specific cellular clone to its micro-environment, predisposing it to creation of mLNs, pointing to a role for LFA-1 in creation of mLNs in CRC.