Comparison of the physical and recombination maps of the mouse X chromosome.
Comparison of the physical and recombination maps of the mouse X chromosome.
复制标题
小鼠 X 染色体物理图和重组图的比较。
DOI：
10.1016/0888-7543(89)90044-x
复制
发表时间：
1989
期刊：
影响因子：
4.4
通讯作者：
Adler,DA
中科院分区：
文献类型：
--
作者：
Disteche,CM;McConnell,GK;Grant,SG;Stephenson,DA;Chapman,VM;Gandy,S;Adler,DA
应用原位杂交技术，对5个随机小鼠基因组DNA标记和5个克隆基因，包括凝血因子VIII和IX（Cf-8和Cf-9）、Duchenne型肌营养不良症（Dmd）、磷酸甘油酸激酶-1（Pgk-1）和α-半乳糖苷酶（Ags）基因在小鼠X染色体上的定位进行了研究。这五个随机DNA标记提供了新的遗传基因座，在小鼠品系和物种之间具有有用的限制性片段长度多态性，包括靠近小鼠X染色体着丝粒区域的一个基因座。比较了这些位点在X染色体上的物理图谱和重组图谱。有很好的协议的顺序位点。基因座之间的相对距离是一致的沿着的X染色体，除了长臂的端粒末端，在物理图谱上密切相关的基因座之间观察到的重组分数高于类似间隔的标记之间位于X染色体的近端区域。这些结果进行了讨论，比较人类X染色体图谱。
The locations of five random mouse genomic DNA markers and five cloned genes, including the genes for clotting factors VIII and IX (Cf-8andCf-9), Duchenne muscular dystrophy (Dmd), phosphoglycerate kinase-1 (Pgk-1), and α-galactosidase (Ags), on the mouse X chromosome were determined byin situhybridization. The five random DNA markers provide new genetic loci with useful restriction fragment length polymorphisms between mouse strains and species, including one locus close to the centromeric region of the mouse X chromosome. The physical map and the recombination map of these loci on the X chromosome were compared. There was good agreement in the order of loci. Relative distances between loci were consistent along the X chromosome, with the exception of the telomeric end of the long arm, where the recombination fraction observed between loci closely associated on the physical map was higher than that between similarly spaced markers located in the proximal region of the X chromosome. These results are discussed in comparison to the human X-chromosome map.