Separation and Analysis of Phenylethanoid Glycosides in Lamiophlomis rotata by High-Performance Liquid Chromatography
Separation and Analysis of Phenylethanoid Glycosides in Lamiophlomis rotata by High-Performance Liquid Chromatography
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DOI：
10.1556/achrom.25.2013.2.12
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发表时间：
2013-06-01
影响因子：
1.9
通讯作者：
Zhao, L.
中科院分区：
文献类型：
--
作者：
Dong, S.;Shi, X.;Zhao, L.
建立了测定独一味中苯乙醇苷类成分含量的简便方法。R.）。不同类型的苯乙醇苷在盐酸溶液中水解生成相应的苯乙醇和肉桂酸，主要含有羟基酪醇、高香子兰醇、3，4-二甲氧基苯乙醇、咖啡酸、富马酸和3，4-二甲氧基肉桂酸。六种分析物可以同时测定的高效液相色谱法（HPLC）。考察了移动的相、运行缓冲液的pH值和浓度、检测波长、流速和进样量等因素对分析的影响。在最佳条件下，6种水解产物在45 min内可完全分离，线性范围为4个数量级，检出限（S/N = 3）为1 × 10 ~（-8）~ 1.5 × 10 ~（-4）mol L ~（-1）。方法用于真实的样品独一味胶囊和奇正炎痛贴剂的分析，结果令人满意。
A simple hydrolysis method has been developed for determination of phenylethanoid glycosides in Lamiophlomis rotata (L. R.). Different kinds of phenylethanoid glycosides were hydrolyzed in hydrochloric acid solution to produce corresponding phenethyl alcohols and cinnamic acids, mainly containing hydroxytyrosol, homovanillyl alcohol, 3,4-dimethoxyphenethyl alcohol, caffeic acid, fumalic acid and 3,4-dimethoxycinnamic acid. The six analytes could be determined simultaneously by high-performance liquid chromatography (HPLC). The effects of mobile phase, pH and concentration of running buffer, detection wavelength, flow rate and injection volume were also investigated. Under the optimum conditions, the six hydrolyzates could be perfectly separated within 45 min. The response was linear over four orders of magnitude with detection limits (S/N = 3) ranging from 1 x 10(-8) to 1.5 x 10(-4) mol L-1 for the analytes. The method has been successfully applied to the analysis of real sample Du-Yi-Wei capsule and Qi-Zheng-Yan-Tong patch, with satisfactory results.