Rapid characterization of complex viscous samples at molecular levels by neutral desorption extractive electrospray ionization mass spectrometry
Rapid characterization of complex viscous samples at molecular levels by neutral desorption extractive electrospray ionization mass spectrometry
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DOI：
10.1038/nprot.2011.337
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发表时间：
2011-06-01
期刊：
影响因子：
14.8
通讯作者：
Chen, Huanwen
中科院分区：
文献类型：
--
作者：
Li, Xue;Hu, Bin;Chen, Huanwen
在此协议中，样品（可以是散装或异质流体，或油腻表面）用中性解吸 (ND) 采样气体束处理，所得分析物混合物直接通过萃取电喷雾电离质谱 (EESI-MS) 进行表征。 ND 设备可以专门构建，使得采样气体束鼓泡通过液体样品（微射流采样）或直接撞击样品表面（例如，用于分析奶酪等材料）。 ND-EESI-MS 分析过程不需要样品预处理，因为它可以承受极其复杂的基质。 ND-EESI-MS 可在环境条件下对高粘度样品进行实时在线化学分析。 ND-EESI-MS 可以轻松检测和定量粘性样品中的挥发性和非挥发性分析物，从而为多个学科（例如食品工业、药物发现以及生物和生命科学）提供基于 MS 的分析平台。在这里，我们描述了用于粘性样品分析的 ND-EESI-MS 协议，包括实验设计、设备设置、试剂制备、数据采集和分析步骤。数据收集过程需要
In this protocol, the sample (which could be a bulk or heterogeneous fluid, or a greasy surface) is treated with a neutral desorption (ND) sampling gas beam, and the resulting analyte mixtures are directly characterized by extractive electrospray ionization mass spectrometry (EESI-MS). The ND device can be specifically constructed such that the sampling gas beam is bubbled through the liquid sample (microjet sampling) or directed to impact the sample surface (e. g., for the analysis of a material like cheese). The ND-EESI-MS analysis process requires no sample pretreatment because it can tolerate an extremely complex matrix. ND-EESI-MS allows real-time, online chemical profiling of highly viscous samples under ambient conditions. Both volatile and nonvolatile analytes from viscous samples can easily be detected and quantified by ND-EESI-MS, thereby providing an MS-based analytical platform for multiple disciplines (e. g., for the food industry, for drug discovery, and for the biological and life sciences). Here we describe the ND-EESI-MS protocol for viscous sample analysis, including the experimental design, equipment setup, reagent preparation, data acquisition and analysis steps. The data collection process takes