The activity of Pax3 and Zic1 regulates three distinct cell fates at the neural plate border
The activity of Pax3 and Zic1 regulates three distinct cell fates at the neural plate border
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DOI：
10.1091/mbc.e06-11-1047
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发表时间：
2007-06-01
影响因子：
3.3
通讯作者：
Saint-Jeannet, Jean-Pierre
中科院分区：
文献类型：
--
作者：
Hong, Chang-Soo;Saint-Jeannet, Jean-Pierre
在非洲爪哇，神经板边缘至少产生三个细胞群：神经脊、胎盘前外胚层和孵化腺。为了了解调控这些谱系形成的分子机制，我们分析了两个转录因子Pax3和Zic1的作用，这两个基因是最早激活的基因之一，对神经板边界诱导信号做出反应。在原肠形成结束时，Pax3和Zic1在神经脊形成区共表达。此外，Pax3在孵化腺的前体细胞中表达，Zic1在胎盘前外胚层中检测到。在整个胚胎和动物外植体中使用功能获得和敲除方法，我们证明了Pax3和Zic1分别是促进孵化腺和胎盘前命运的必要条件和充分条件，而它们的联合活性对于指定神经峰是必不可少的。此外，我们还表明，通过控制Pax3和Zic1的水平，有可能在这些细胞之间改变命运。这些发现为调节神经板边界细胞命运决定的机制提供了新的信息。
In Xenopus, the neural plate border gives rise to at least three cell populations: the neural crest, the preplacodal ectoderm, and the hatching gland. To understand the molecular mechanisms that regulate the formation of these lineages, we have analyzed the role of two transcription factors, Pax3 and Zic1, which are among the earliest genes activated in response to neural plate border-inducing signals. At the end of gastrulation, Pax3 and Zic1 are coexpressed in the neural crest forming region. In addition, Pax3 is expressed in progenitors of the hatching gland, and Zic1 is detected in the preplacodal ectoderm. Using gain of function and knockdown approaches in whole embryos and animal explants, we demonstrate that Pax3 and Zic1 are necessary and sufficient to promote hatching gland and preplacodal fates, respectively, whereas their combined activity is essential to specify the neural crest. Moreover, we show that by manipulating the levels of Pax3 and Zic1 it is possible to shift fates among these cells. These findings provide novel information on the mechanisms regulating cell fate decisions at the neural plate border.