Identification of 86 candidates for small non-messenger RNAs from the archaeon Archaeoglobus fulgidus
Identification of 86 candidates for small non-messenger RNAs from the archaeon Archaeoglobus fulgidus
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DOI：
10.1073/pnas.112047299
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发表时间：
2002-05-28
影响因子：
11.1
通讯作者：
Hüttenhofer, A
中科院分区：
文献类型：
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作者：
Tang, TH;Bachellerie, JP;Hüttenhofer, A
在考古Archaeoglobus fulgidus的专门cDNA文库中，我们确定了总共有86个不同表达的RNA序列，这些序列可能编码先前未表征的小型非允许人RNA（SNMRNA）物种。这些RNA中的十种类似于真核小核RNA，它们引导rRNA 2'-O-甲基化（C/D-box型）和伪苷（H/ACA-box型）。因此，我们在古细菌物种中确定了H/ACA小RNA的四个候选者，这些候选者被预测将指导总共六个rRNA伪苷。此外，我们已经通过实验验证了六种预测的伪基的存在。我们证明了22个snmrnas是从一个短串联重复的家族中转录的，保守了内部的古细胞基因组，并以前可能与复制子分配有关。此外，鉴定出源自fulgidus的rRNA操纵子的四个SNMRNA被鉴定出来，并证明是通过前RRNA的剪接/处理途径产生的。由于缺乏已知的结构和/或序列基序，其余50个RNA无法分配到已知的SNMRNA。关于它们在基因组上的位置，只有9个位于基因间区域，而33个是与ORF互补的，其中5个重叠一个ORF，而3个来自ORF内的感官方向。我们的研究进一步支持了SNMRNA在生命的所有三个领域中的重要性。
In a specialized cDNA library from the archaeon Archaeoglobus fulgidus we have identified a total of 86 different expressed RNA sequences potentially encoding previously uncharacterized small non-messenger RNA (snmRNA) species. Ten of these RNAs resemble eukaryotic small nucleolar RNAs, which guide rRNA 2'-O-methylations (C/D-box type) and pseudouridylations (H/ACA-box type). Thereby, we identified four candidates for H/ACA small RNAs in an archaeal species that are predicted to guide a total of six rRNA pseudouridylations. Furthermore, we have verified the presence of the six predicted pseudouridines experimentally. We demonstrate that 22 snmRNAs are transcribed from a family of short tandem repeats conserved inmost archaeal genomes and shown previously to be potentially involved in replicon partitioning. In addition, four snmRNAs derived from the rRNA operon of A. fulgidus were identified and shown to be generated by a splicing/processing pathway of pre-rRNAs. The remaining 50 RNAs could not be assigned to a known class of snmRNAs because of the lack of known structure and/or sequence motifs. Regarding their location on the genome, only nine were located in intergenic regions, whereas 33 were complementary to an ORF, five were overlapping an ORF, and three were derived from the sense orientation within an ORF. Our study further supports the importance of snmRNAs in all three domains of life.