CELL STRUCTURE IN TREE RINGS: NOVEL METHODS FOR PREPARATION AND IMAGE ANALYSIS OF LARGE CROSS SECTIONS

CELL STRUCTURE IN TREE RINGS: NOVEL METHODS FOR PREPARATION AND IMAGE ANALYSIS OF LARGE CROSS SECTIONS
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年轮中的细胞结构:大横截面制备和图像分析的新方法

DOI:
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发表时间:
2000
期刊:
影响因子:
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通讯作者:
W. Doll
W. Doll
中科院分区:
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文献类型:
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作者:
H. Spiecker;M. Schinker;J. Hansen;Young;T. Ebding;W. Doll

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本文介绍了一种新的用于高分辨率反射光显微镜的木材样品制备方法,该方法结合了一种种特定的图像分析系统。该方法是基于一个超精密单点金刚石飞刀配备空气轴承和功能的基础上旋转的金刚石边缘。可以在增量型芯和整个横截面上生产广泛且非常光滑的表面。因此,这种技术提供了一个连续的显微镜分析的先决条件,在整个大年轮序列,或整个横截面。不需要修补或分段。云杉和橡树的细胞结构的高分辨率分析介绍了新的方法来描述和预测直径增长的各种环境事件的反应。
A new method for the preparation of wood samples for high-resolution reflected-light microscopy in combination with a species-specific image analysis system is introduced. The method is based on an ultraprecise single-point diamond flycutter equipped with air bearings and functions on the basis of a rotating diamond edge. Extensive and very smooth surfaces can be produced on increment cores as well as entire cross sections. Hence, this technique provides the prerequisites for a continuous microscopic analysis across large tree-ring sequences, or entire cross sections. Patching or sub-sectioning is not required. The high-resolution analysis of cell structures of spruce and oak presented here introduces new approaches for describing and predicting reactions of diameter growth to various environmental events.