Mapping of Redox Status in a Brain-Disease Mouse Model by Three-Dimensional EPR Imaging
Mapping of Redox Status in a Brain-Disease Mouse Model by Three-Dimensional EPR Imaging
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DOI：
10.1002/mrm.22598
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发表时间：
2011-01-01
影响因子：
3.3
通讯作者：
Hirata, Hiroshi
中科院分区：
文献类型：
--
作者：
Fujii, Hirotada;Sato-Akaba, Hideo;Hirata, Hiroshi
利用氮氧化物进行电子顺磁共振成像是一种观察体内氧化应激调节的氧化还原状态的有效方法。然而，当在小型啮齿动物中使用连续波电子顺磁共振成像仪时，使用具有相对较短的T(2)和半衰期值的氮氧化物时，通常数据采集时间太慢，无法获得足够数量的三维图像投影。由于成像仪的改进，使快速数据采集成为可能，在小鼠体内用氮氧化物测试了高质量三维电子顺磁共振成像的可行性。在强电子顺磁共振信号下，每隔15秒场扫描0.3秒获得小鼠的三维图像，在强电子顺磁共振信号下有46个投影。3-羟甲基-2,2,5,5-四甲基吡咯烷-1-氧基在小鼠脑内的三维电子顺磁共振成像显示，3-羟甲基-2,2,5,5-四甲基吡咯烷-1-氧基分布在脑组织内，并通过MRI观察证实了这一点。基于一氧化氮在小鼠体内的药代动力学，在小鼠脑缺血再灌注模型中证实了半衰期作图。利用改进的连续波电子顺磁共振成像技术，对氧化应激下小鼠头部的非均匀半衰期进行了逐像素的清晰成像。中国生物医学工程杂志（英文版），2011。(C) 2010 Wiley-Liss, Inc。
Electron paramagnetic resonance imaging using nitroxides is a powerful method for visualizing the redox status modulated by oxidative stress in vivo. Typically, however, data acquisition times have been too slow to obtain a sufficient number of projections for three-dimensional images, when using continuous wave-electron paramagnetic resonance imager in small rodents, using nitroxides with comparatively short T(2) and a half-life values. Because of improvements in imagers that enable rapid data-acquisition, the feasibility of three-dimensional electron paramagnetic resonance imaging with good quality in mice was tested with nitroxides. Three-dimensional images of mice were obtained at an interval of 15 sec under field scanning of 0.3 sec and with 46 projections in the case of strong electron paramagnetic resonance signals. Three-dimensional electron paramagnetic resonance images of a blood brain barrier-permeable nitroxide, 3-hydroxymethyl-2,2,5,5- tetramethylpyrrolidine-1-oxyl, in the mouse head clearly showed that 3-hydroxymethyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl was distributed within brain tissues, and this was confirmed by MRI observations. Based on the pharmacokinetics of nitroxides in mice, half-life mapping was demonstrated in an ischemia-reperfusion model mouse brain. Inhomogeneous half-lives were clearly mapped pixel-by-pixel in mouse head under oxidative stress by the improved continuous wave-electron paramagnetic resonance imager noninvasively. Magn Reson Med 65: 295-303, 2011. (C) 2010 Wiley-Liss, Inc.