LUTEOTROPHIC AND LUTEOLYTIC INTERACTIONS BETWEEN BOVINE SMALL AND LARGE LUTEAL-LIKE CELLS AND ENDOTHELIAL-CELLS
LUTEOTROPHIC AND LUTEOLYTIC INTERACTIONS BETWEEN BOVINE SMALL AND LARGE LUTEAL-LIKE CELLS AND ENDOTHELIAL-CELLS
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DOI：
10.1095/biolreprod52.4.954
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发表时间：
1995-04-01
影响因子：
3.6
通讯作者：
MEIDAN, R
中科院分区：
文献类型：
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作者：
GIRSH, E;GREBER, Y;MEIDAN, R
内皮细胞是牛CL中最丰富的细胞类型，可与CL的类固醇生成细胞建立细胞间接触。两种实验模型用于研究内皮细胞参与黄体细胞功能：1）维持不同细胞之间的完整性和通信的黄体切片和2）单独培养或与内皮细胞共培养的纯的大和小黄体样细胞。在这两种模型中检查了前列腺素（PG）F-2 α的溶黄体作用。用PGF（2 α）处理未改变年轻（2-4天）CL切片中LH刺激的P-4分泌，而在成熟（6-12天）CL切片中，PGF（2 α）显著降低（40%）LH对P-4分泌的刺激作用。在纯的大黄体样细胞中，孵育3或24小时后，毛喉素加PGF（2 α）对P-4分泌的影响与单独给予毛喉素没有差异。然而，当与内皮细胞共培养时，PGF（2 α）显著抑制毛喉素刺激。内皮细胞显着刺激P-4生产大黄体样细胞。这种作用可能是由于内皮细胞分泌的PGI（2）的作用。总之，内皮细胞可能通过参与促黄体和溶黄体过程而在黄体功能中发挥重要作用。
Endothelial cells, the most abundant cell type in the bovine CL, were shown to establish intercellular contact with steroidogenic cells of the CL. Two experimental models were used to study the involvement of endothelial cells in luteal cell function: 1) luteal slices in which the integrity and communication between the different cells were maintained and 2) pure large and small luteal-like: cells, cultured separately or co-cultured with endothelial cells. The luteolytic effect of prostaglandin (PG) F-2 alpha was examined in these two models. Treatment with PGF(2 alpha) did not alter P-4 secretion stimulated by LH in young (2-4-day-old) CL slices, whereas, in slices from mature (6-12 days old) CL, PGF(2 alpha) significantly reduced (by 40%) the stimulatory effect of LH on P-4 secretion. In pure large luteal-like cells, the effect of forskolin plus PGF(2 alpha) on P-4 secretion did not differ from forskolin given alone after 3 or 24 h of incubation. However, when co-cultured with endothelial cells, PGF(2 alpha) significantly inhibited forskolin stimulation. Endothelial cells significantly stimulated P-4 production from large luteal-like cells only. This effect may be attributed to the action of PGI(2) secreted by endothelial cells.In summary, endothelial cells may play an essential role in luteal functions by being involved in both luteotrophic and luteolytic processes.