Epithelia-derived wingless regulates dendrite directional growth of drosophila ddaE neuron through the Fz-Fmi-Dsh-Rac1 pathway.
Epithelia-derived wingless regulates dendrite directional growth of drosophila ddaE neuron through the Fz-Fmi-Dsh-Rac1 pathway.
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上皮源性 wingless 通过 Fz-Fmi-Dsh-Rac1 通路调节果蝇 ddaE 神经元的树突定向生长
DOI：
10.1186/s13041-016-0228-0
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发表时间：
2016-04-29
期刊：
影响因子：
3.6
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Li X;Wang Y;Wang H;Liu T;Guo J;Yi W;Li Y
背景：正确的树突构型对于神经系统中信息的接收和处理是至关重要的。细胞自主分子在树突形态发生中已经得到了广泛的研究，但环境因素对树突生长的调控机制尚不清楚。结果：通过评估两个初级树突之间的夹角(PD角)，我们发现果蝇周围感觉神经元ddaE的初级树突的定向生长受形态分子Wingless(Wg)的调控。在树突生长的早期，Wg表达于ddaE后面的一组上皮细胞。当Wg表达减少或前移时，PD角明显减小。此外，Wg受体FrizzledWg与Flamingo共同参与Wg信号向ddaE神经元的传导，其下游信号通过Rac1通过非典范Wnt途径传递。结论：提示上皮源性Wg通过非典范Wnt途径在调节树突的定向生长中起排斥作用。因此，我们的发现为环境信号如何作为树突图案的空间线索提供了强有力的活体证据。
Background:Proper dendrite patterning is critical for the receiving and processing of information in the nervous system. Cell-autonomous molecules have been extensively studied in dendrite morphogenesis; however, the regulatory mechanisms of environmental factors in dendrite growth remain to be elucidated.Results:By evaluating the angle between two primary dendrites (PD-Angle), we found that the directional growth of the primary dendrites of a Drosophila periphery sensory neuron ddaE is regulated by the morphogen molecule Wingless (Wg). During the early stage of dendrite growth, Wg is expressed in a group of epithelial cells posteriorly adjacent to ddaE. When Wg expression is reduced or shifted anteriorly, the PD-Angle is markedly decreased. Furthermore, Wg receptor Frizzled functions together with Flamingo and Dishevelled in transducing the Wg signal into ddaE neuron, and the downstream signal is mediated by non-canonical Wnt pathway through Rac1.Conclusions:In conclusion, we reveal that epithelia-derived Wg plays a repulsive role in regulating the directional growth of dendrites through the non-canonical Wnt pathway. Thus, our findings provide strong in vivo evidence on how environmental signals serve as spatial cues for dendrite patterning.