Bovine babesiosis:: Anti-erythrocyte antibodies purification from the sera of naturally infected cattle
Bovine babesiosis:: Anti-erythrocyte antibodies purification from the sera of naturally infected cattle
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DOI：
10.1016/j.vetimm.2006.12.011
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发表时间：
2007-04-15
影响因子：
1.8
通讯作者：
Gontijo, C. M.
中科院分区：
文献类型：
--
作者：
Goes, T. S.;Goes, V. S.;Gontijo, C. M.
巴贝斯虫和牛巴贝斯虫是红细胞体内的原生动物寄生虫，通过扁虱传播给牛，在牛身上引发巴贝斯虫病，一种类似人类疟疾的疾病。贫血是由未感染的红细胞破坏引起的，是该病的一个重要特征。抗红细胞抗体可能是造成这种破坏的原因之一。这些抗体在分别感染吉氏杆菌和罗德海尼杆菌的狗和老鼠的血清中发现。然而，关于感染牛的血清中存在抗红细胞抗体的数据并不是决定性的。在本研究中，我们试图从血清中检测一种抗红细胞抗体。指自然感染双链球菌的牛。用一头未感染的小牛的红细胞进行酶联免疫吸附试验，检测样品中的抗体。结果证实，感染牛血清中存在较高水平的抗红细胞抗体。为了将这一增量与寄生虫联系起来，从感染的小牛血清中提纯了抗红细胞抗体，连接到Sepharose4B柱上，然后用于抗独特型抗体的纯化。这些抗体被发现与寄生虫发生反应，这表明抗寄生虫抗体和抗红细胞抗体之间存在相关性。(C)2007 Elsevier B.V.保留所有权利。
Babesia bigemina and Babesia bovis are intra-erythrocytic protozoan parasites transmitted by ticks to cattle in which they induce babesiosis, a disease that resembles human malaria. Anemia, caused by the destruction of non-infected erythrocytes, is a critical feature of the disease. Anti-erythrocyte antibodies could be one of the explanations for such destruction. These antibodies are found in the sera of dogs and mice respectively infected with B. gibsoni and B. rodhaini. However, data concerning the presence of anti-erythrocyte antibodies in the sera of infected cattle are not conclusive. In the present study, we made an attempt to detect aanti-erythrocyte antibodies from the sera. of cattle naturally infected with B. bigemina. Erythrocytes from a non-infected calf were used in ELISA reaction for the detection of antibodies from samples. Results confirmed the presence of anti-erythrocytes antibodies in higher amounts in the serum of infected cattle. In order to correlate this increment with the parasite, anti-erythrocyte antibodies from the sera from infected calves were purified, coupled to a Sepharose-4B column and than used for anti-idiotypic antibodies purification. These antibodies were found to react with the parasites, suggesting a correlation between both anti-parasite and anti-erythrocyte antibodies. (c) 2007 Elsevier B.V. All rights reserved.