Preferential expansion of Vγ9 Vδ2 T cells following stimulation of peripheral blood lymphocytes with extracts of Plasmodium falciparum
Preferential expansion of Vγ9 Vδ2 T cells following stimulation of peripheral blood lymphocytes with extracts of Plasmodium falciparum
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用恶性疟原虫提取物刺激外周血淋巴细胞后 Vγ9 Vδ2 T 细胞优先扩增
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
P. Dubois
中科院分区：
文献类型：
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作者：
C. Behr;P. Dubois
先前已描述了恶性疟原虫（Plasmodium falciparum）抗原裂解物是人外周T淋巴细胞增殖的强力诱导剂。在这份报告中，我们研究了未暴露供体的循环T细胞的表型，并研究了恶性疟原虫裂解物与传统T细胞有丝分裂原植物血凝素（PHA），一种已知的超抗原葡萄球菌肠毒素B（SE B）和一种经典的抗原纯蛋白衍生物（PPD）的比较。我们表明，这种淋巴增殖活性的相互作用与MHC II类分子是必需的，并在恶性疟原虫的存在下，绝大多数的循环细胞在第6天是γ δ T细胞，所有这些轴承V γ 9 V δ 2。我们的研究结果表明，恶性疟原虫诱导T细胞增殖反应，类似于人外周血γ δ T细胞对超抗原的反应。这一观察结果与疟疾急性感染期间和之后观察到的外周γ δ淋巴细胞水平升高一致。
A Plasmodium falciparum schizont lysate has been previously described as being a powerful inducer of proliferation for human peripheral T lymphocytes. In this report we study the phenotype of cycling T cells from unexposed donors and examine how the P. falciparum lysate compares with the conventional T cell mitogen phytohemagglutinin (PHA), a known superantigen staphylococcal enterotoxin B (SEB), and a classical antigen pure protein derivative (PPD). We show that for this lymphoproliferative activity interaction with the MHC class II molecule is required and that in the presence of P. falciparum the great majority of the cycling cells at day 6 are gamma delta T cells, all of them bearing V gamma 9 V delta 2. Our results suggest that P. falciparum induces a T cell proliferative response that resembles a response of human peripheral blood gamma delta T cells to superantigen. This observation is in agreement with the elevated level of peripheral gamma delta lymphocytes observed during and after malaria acute infection.