GENETICS OF USTILAGO-VIOLACEA .5. OUTCROSSING AND SELFING IN TELIOSPORE INOCULA
GENETICS OF USTILAGO-VIOLACEA .5. OUTCROSSING AND SELFING IN TELIOSPORE INOCULA
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DOI：
10.1086/337061
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发表时间：
1979-01-01
影响因子：
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通讯作者：
GARBER, ED
中科院分区：
文献类型：
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作者：
BAIRD, ML;GARBER, ED
用4株具有不同菌落颜色、不同营养不良标记和不同交配类型的紫紫堇单倍体孢子菌悬浮液接种白硅烯植株，产生的端孢仅含有3种营养不良基因型中的1种。每株植物的所有被测芽都具有相同基因型的端孢子。用杂合的端孢子作为菌落颜色标记接种的每一株的黑穗病芽通常产生具有相同基因型的端孢子。不同菌落颜色基因型的端孢混合接种的异交率为6.6%。用杂合子端孢子接种的黑穗病植株中，有4.6%获得纯合子端孢子作为颜色标记。利用孢子克隆的基因型检测了传染性双核菌丝细胞核中一个颜色标记的自发突变。这些观察结果表明，新的生理小种可能是由双核菌丝的自发突变产生的，产生杂合的端孢子，最终通过自交产生重组的端孢子。
Plants of Silene alba inoculated with a suspension of 4 haploid sporidial strains of U. violacea with different colony color and auxotrophic markers and mating types yielded teliospores with only 1 of the 3 possible auxotrophic genotypes. All of the tested buds from each plant had teliospores with the same genotype. Smutted buds from each plant inoculated with teliospores heterozygous for colony color markers usually gave teliospores with the same genotype. The frequency of outcrossing in a mixed inoculum of teliospores with different colony color genotypes was 6.6%. Homozygous teliospores were obtained from 4.6% of the smutted plants inoculated with heterozygous teliospores for color markers. Spontaneous mutation for a color marker in the nuclei of infectious dikaryotic hyphae was detected by the genotype of sporidial clones in colonies from teliospores. These observations suggest that new physiological races in the species may result from spontaneous mutation in dikaryotic hyphae, yielding heterozygous teliospores which can eventually produce recombinant teliospores by selfing.