Trypanosoma cruzi:: Impact of clonal evolution of the parasite on its biological and medical properties
Trypanosoma cruzi:: Impact of clonal evolution of the parasite on its biological and medical properties
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DOI：
10.1006/expr.1998.4216
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发表时间：
1998-05-01
影响因子：
2.1
通讯作者：
Tibayrenc, M
中科院分区：
文献类型：
--
作者：
Revollo, S;Oury, B;Tibayrenc, M
克氏锥虫种群被细分为可以表现出相当大的遗传差异的自然克隆。有研究认为克氏绦虫克隆结构对其生物学特性有重要影响。目前的工作旨在验证这一假设。采用22个遗传位点的多位点酶电泳（MLEE）和10条引物随机扩增的多态性DNA (RAPD)，以及14种不同的生物学参数，对从不同生态循环、不同地点和不同寄主中分离的21个种群进行了特征分析。这些参数与：(1)附体和无尾体的生长动力学有关；（ii）培养细胞被无尾线虫感染；（iii）细胞外锥乳线虫的生存能力；或（iv）拟马鞭毛虫、锥马鞭毛虫和无尾鞭毛虫对苯并硝唑和硝呋替莫的敏感性。MLEE和RAPD结果基本一致，如前面所述（M. Tibayrenc， K. Neubauer， C. Barnabe， F. Guerrini， D. Skarecky, and F. J. Ayala, 1993, Proceedings of National Academy of Sciences of the us490,1335 -1339），并表明21个种群分布在19/ 20,32和39三个主要遗传群中，对应于先前基于15个同位酶位点描述的19、20、32和39个主要克隆。大多数生物学参数显示与MLEE或RAPD评估的遗传距离有很强的相关性，这有利于工作假设。唯一的例外是对锥马鞭毛虫的药物敏感性。总体结果使我们有可能坚定地拒绝零假设，即克氏T. cruzi自然克隆的进化距离和生物学差异之间没有关系。(C) 1998学术出版社。
Trypanosoma cruzi populations are subdivided into natural clones that can exhibit considerable genetic differences. It has been proposed that T. cruzi clonal structure has a major impact on this parasite's biological properties. The present work aims at testing this hypothesis. Twenty-one stocks isolated from various ecological cycles, places, and hosts were characterized by multilocus enzyme electrophoresis (MLEE) with 22 genetic loci and random amplification of polymorphic DNA (RAPD) with 10 primers on the one hand and by 14 different biological parameters on the other hand. These parameters were related to: (i) growth kinetics of epimastigotes and amastigotes; (ii) infection of culture cells by amastigotes; (iii) viability of extracellular trypomastigotes; or (iv) sensitivity of epimastigotes, trypomastigotes, and amastigotes to Benznidazole and Nifurtimox. MLEE and RAPD results exhibited parity to each other, as previously noted (M. Tibayrenc, K. Neubauer, C. Barnabe, F. Guerrini, D. Skarecky, and F. J. Ayala, 1993, Proceedings of the National Academy of Sciences of the US4 90, 1335-1339), and showed that the 21 stocks were distributed into three main genetic groups, 19/20, 32, and 39, corresponding to the major clones 19, 20, 32, and 39 previously described on the basis of 15 isozyme loci. Most biological parameters showed a strong correlation to the genetic distances evaluated from either MLEE or RAPD, which favors the working hypothesis. The only exception came from drug sensitivity estimated on trypomastigote forms. The overall results made it possible to firmly reject the null hypothesis that there is no relationships between evolutionary distances and biological differences in T. cruzi natural clones. (C) 1998 Academic Press.