Nanosecond Pulse Generator with Variable Pulse Duration for the Study of Pulse Induced Biological Effects
Nanosecond Pulse Generator with Variable Pulse Duration for the Study of Pulse Induced Biological Effects
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用于研究脉冲引起的生物效应的具有可变脉冲持续时间的纳秒脉冲发生器
DOI：
10.1109/ipmc.2008.4743577
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
L. Zeni
中科院分区：
文献类型：
--
作者：
J. Kolb;S. Scarlett;J. Cannone;J. Zhuang;C. Osgood;K. Schoenbach;A. de Angelis;L. Zeni
我们已经开发了一种Blumlein线脉冲发生器，它利用从两个单独的开关脉冲形成线发射的电脉冲的叠加。通过使用快速功率MOSFET作为Blumlein线两端的开关，我们能够在100 Ω负载上产生幅度为1 kV的脉冲。脉冲持续时间和极性可以通过两个开关的触发中的时间延迟来控制。此外，使用相同的开关，使我们能够克服在传统的Blumlein配置中使用非理想开关所产生的脉冲失真。利用该脉冲发生器，将持续时间在8和300 ns之间的脉冲施加到Jurkat细胞（白血病细胞系）以研究钙水平的脉冲依赖性增加。通过使用钙指示剂fluo-4的旋转圆盘共聚焦荧光显微镜研究单个细胞中钙水平的发展。与此快速成像系统，荧光的变化，代表钙动员，可以解决5毫秒，每18毫秒的曝光。对于60纳秒的脉冲持续时间，细胞内钙的每一个上升更大的电场强度从25千伏/厘米增加到100千伏/厘米。仅对于最高电场强度，响应依赖于细胞外钙的存在。结果补充离子交换机制之前观察到的充电过程中的细胞膜，这是建议在曝光过程中的膜电位变化的观察。
We have developed a Blumlein line pulse generator which utilizes the superposition of electrical pulses launched from two individually switched pulse forming lines. By using a fast power MOSFET as a switch on each end of the Blumlein line, we were able to generate pulses with amplitudes of 1 kV across a 100-Omega load. Pulse duration and polarity can be controlled by the temporal delay in the triggering of the two switches. In addition, the use of identical switches allows us to overcome pulse distortions arising from the use of non-ideal switches in the traditional Blumlein configuration. With this pulse generator, pulses with durations between 8 and 300 ns were applied to Jurkat cells (a leukemia cell line) to investigate the pulse dependent increase in calcium levels. The development of the calcium levels in individual cells was studied by spinning-disc confocal fluorescent microscopy with the calcium indicator, fluo-4. With this fast imaging system, fluorescence changes, representing calcium mobilization, could be resolved with an exposure of 5 ms every 18 ms. For a 60-ns pulse duration, each rise in intracellular calcium was greater as the electric field strength was increased from 25 kV/cm to 100 kV/cm. Only for the highest electric field strength is the response dependent on the presence of extracellular calcium. The results complement ion-exchange mechanisms previously observed during the charging of cellular membranes, which were suggested by observations of membrane potential changes during exposure.
影响因子：
3.4
作者：
Frey, W;White, JA;Kolb, JF
通讯作者：
Kolb, JF