Uterus transplantation in the rat: Model development, surgical learning and morphological evaluation of healing
Uterus transplantation in the rat: Model development, surgical learning and morphological evaluation of healing
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DOI：
10.1080/00016340802484966
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发表时间：
2008-01-01
影响因子：
4.3
通讯作者：
Brannstrom, Mats
中科院分区：
文献类型：
--
作者：
Wranning, Caiza A.;Akhi, Shamina N.;Brannstrom, Mats
目标。实验性子宫移植是一个不断发展的研究领域，其目的是开发一种治疗女性子宫绝对因素不孕的方法。在临床试验开始之前，需要在适当的动物模型中识别和最小化子宫移植手术和免疫抑制治疗的潜在风险。本研究的目的是建立和评估大鼠子宫移植模型，该模型可用于移植后子宫功能关键方面的未来研究。设计。动物研究。设置。大学医院。样本。大鼠非排斥子宫移植术后不同时间点的子宫组织取样。方法。成年、未交配的Lewis株雌性大鼠分别作为子宫移植的供体和受体。两名没有受过显微外科训练的人进行了移植，并记录了学习曲线。当两名外科医生的移植存活率均超过70%时，移植15只动物，并在术后1、7和21天对移植子宫进行形态学评估和浸润中性粒细胞计数。主要结果测量。动物存活率，移植物存活率，手术次数，子宫形态，浸润中性粒细胞计数。结果。两位外科医生都获得了必要的显微外科技术，以相似的比率达到70%以上的移植存活率。移植后第一天的术后炎症症状较轻，在以后的时间点进一步减轻。结论。建立了一种可重复的大鼠子宫移植模型，为进一步研究同种异体子宫移植后的子宫功能奠定了基础。
Objective. Experimental uterus transplantation is a growing research field with the aim to develop a treatment for women with absolute uterus factor infertility. The potential risks of surgery and immunosuppressive treatment involved in uterus transplantation need to be identified and minimized in appropriate animal models before clinical trials commence. The aim of the present study was to develop and evaluate a model for uterus transplantation in the rat that can be reproduced and used in future studies concerning critical aspects of uterine function after transplantation. Design. Animal study. Setting. University Hospital. Sample. Uterine tissue sampled at different post-operative time points after non-rejecting uterus transplantation in rats. Methods. Adult, virgin female rats of inbred Lewis strain served as donors and recipients of uterine transplants. Two individuals with no previous microsurgical training performed the transplantations and learning curves were recorded. When transplant survival exceeded 70% for both surgeons, 15 animals were transplanted and grafted uteri were evaluated at 1, 7 and 21 days after surgery by assessment of morphology and enumeration of infiltrating neutrophilic granulocytes. Main outcome measures. Animal survival, graft survival, surgery times, uterine morphology, enumeration of infiltrating neutrophilic granulocytes. Results. Both surgeons gained the necessary microsurgical skills needed to achieve above 70% transplant survival at a similar rate. The signs of post-operative inflammation on day one after transplantation were minor and further reduced at later time points. Conclusion. A reproducible model for uterus transplantation in the rat was developed, which can be used in future studies concerning uterine function after allogenic transplantation.