Constitutive expression of Cry proteins in roots and border cells of transgenic cotton
Constitutive expression of Cry proteins in roots and border cells of transgenic cotton
复制标题
DOI：
10.1007/s10681-006-9272-7
复制
发表时间：
2007-03-01
期刊：
影响因子：
1.9
通讯作者：
Stiller, Warwick N.
中科院分区：
文献类型：
--
作者：
Knox, Oliver G. G.;Gupta, Vadakattu V. S. R.;Stiller, Warwick N.
转Bt基因棉花对某些鳞翅目害虫有较好的防治效果，但其地下表达的蛋白质却知之甚少。我们使用ELISA定量在体外表达的Cry 1Ac和Cry 2Ab蛋白质的粘液，根边缘细胞和根尖在5个转基因棉花品种相比，常规品种Sicot 189。Cry蛋白在转基因棉花品种的根和边缘细胞中的表达是组成性的并且处于可检测的水平，其中Cry 1Ac和Cry 2Ab蛋白表达范围为< 20 ppb to >100 pbb。为了确定转基因棉花与优良亲本系（cv. Sicot 189），对胚根边缘细胞进行了计数。14个品种根尖边缘细胞数为0.2 ~ 1.1 × 10 ~（4）个，平均5 × 10 ~（3）个。边缘细胞生产的转基因品种一般是类似的供体和精英的父母，例外的是品种Sicot 189，它有更多的边缘细胞比所有的转基因衍生物。边缘细胞数与品种抗病性等级的比较发现，边缘细胞数与棉花枯萎病抗性等级之间存在有限的相关性（r（2）=0.65，n=7）。棉花品种边缘细胞数量和根尖Cry蛋白表达的差异对根际和土壤生态系统中植物-微生物相互作用的影响尚未得到解决。
Transgenic cotton plants expressing Cry1Ac and Cry2Ab, from the soil bacterium Bacillus thuringiensis (Bt), provide effective control of certain lepidopteran pests, however, little is known about the proteins below ground expression. We used ELISA to quantify in vitro expression of the Cry1Ac and Cry2Ab proteins in mucilage, root border cells and root tips in five transgenic cultivars of cotton compared to conventional cultivar Sicot 189. Expression of Cry proteins in roots and border cells of the transgenic cotton cultivars was constitutive and at detectable levels, with Cry1Ac and Cry2Ab protein expression ranging from < 20 ppb to > 100 pbb. To determine if genetically modified cotton demonstrated simple differences in properties of the root, when compared to an elite parental line (cv. Sicot 189), we enumerated border cells on seedling radicles. Border cell counts of 14 cultivars ranged from 0.2 to 1.1 x 10(4) cells per root tip with an average of 5 x 10(3) border cells. Border cell production in the transgenic cultivars was generally similar to that of both donor and elite parents, the exception being the cultivar Sicot 189, which had substantially more border cells than all of its transgenic derivatives. Comparison of border cell number with varietal disease resistance ranking found a limited relationship (r(2) =0.65, n=7) between border cell numbers and the commercial resistance rank against Fusarium wilt of cotton. The implications of differences in cotton cultivar border cell number and root tip expression of Cry proteins for plant-microbe interactions in the rhizosphere and the soil ecosystem are yet to be resolved.