Biomimetic-Engineered Poly (Ethylene Glycol) Hydrogel for Smooth Muscle Cell Migration
Biomimetic-Engineered Poly (Ethylene Glycol) Hydrogel for Smooth Muscle Cell Migration
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DOI：
10.1089/ten.tea.2013.0050
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发表时间：
2014-02-01
影响因子：
4.1
通讯作者：
Marchant, Roger E.
中科院分区：
文献类型：
--
作者：
Lin, Lin;Zhu, Junmin;Marchant, Roger E.
我们报告的仿生支架作为模型系统，以评估平滑肌细胞（SMC）迁移的三维。为了实现这一点，生物惰性聚（乙二醇）（PEG）为基础的水凝胶被设计为支架基板。为了模拟细胞外基质的性质，将源自纤连蛋白的细胞粘附肽（GRGDSP）和源自I型胶原的胶原酶敏感肽（GPQGIAGQ）并入PEG大分子单体链中。仿生大分子单体的共聚导致形成具有细胞粘附性和生物降解性的生物活性PEG水凝胶。通过利用这些仿生支架，我们研究了粘附配体浓度，蛋白水解和网络交联密度对细胞迁移的影响。我们的研究结果表明，三维SMC迁移有一个双相依赖于粘附配体密度，和粘附和胶原酶敏感的肽细胞迁移发生所需的。此外，网络交联密度显着影响细胞在水凝胶中的迁移行为。
We report on a biomimetic scaffold as a model system to evaluate smooth muscle cell (SMC) migration in three dimensions. To accomplish this, bio-inert poly (ethylene glycol) (PEG)-based hydrogels were designed as the scaffold substrate. To mimic properties of the extracellular matrix, cell-adhesive peptide (GRGDSP) derived from fibronectin and collagenase-sensitive peptide (GPQGIAGQ) derived from collagen type I were incorporated into the PEG macromer chain. Copolymerization of the biomimetic macromers results in the formation of bioactive PEG hydrogels with cell adhesivity and biodegradability. By utilizing these biomimetic scaffolds, we studied the effect of adhesive ligand concentration, proteolysis, and network cross-linking density on cell migration. Our results showed that three-dimensional SMC migration has a biphasic dependence on adhesive ligand density, and both adhesive and collagenase-sensitive peptides were required for cell migration to occur. Furthermore, network cross-linking density was shown to dramatically influence the behavior of cell migration in the hydrogels.