The expression of gill Na, K-ATPase in milkfish, Chanos chanos, acclimated to seawater, brackish water and fresh water
The expression of gill Na, K-ATPase in milkfish, Chanos chanos, acclimated to seawater, brackish water and fresh water
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DOI：
10.1016/s1095-6433(03)00136-3
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发表时间：
2003-07-01
影响因子：
2.3
通讯作者：
Lee, TH
中科院分区：
文献类型：
--
作者：
Lin, YM;Chen, CN;Lee, TH
将Chanos chanos（Forssk（a）over circle 1，1775）从当地养鱼场转移到实验室的淡水（FW; 0 ppm）、半咸水（BW，10 ppm，20 ppm）和海水（SW; 35 ppm）条件下饲养至少两周。对适应不同盐度的鱼的血液和鳃进行了分析，以确定这种广盐性物种的盐度调节能力。适应不同盐度的遮鱼血浆渗透压、钠和氯浓度无显著差异。鳃Na，K-ATPase（NKA）比活性以FW组最高，SW组最低。鳃NKA α-亚基的相对丰度揭示了类似的配置文件。然而，与其他广盐性硬骨鱼，即罗非鱼，鲑鱼和鳗鱼相反，自然SW居住的遮流鱼在BW和FW中表达更高的NKA活性。免疫细胞化学染色显示，适应BW和SW的鱼的Na，K-ATP酶免疫反应（NKIR）细胞主要分布在肌丝上，板层上的NKIR细胞很少。此外，在适应FW的遮流鱼中，在板状体上发现的NKIR细胞的数量显著增加。低盐环境下，使NKIR细胞数量和NKA活性升高的反应被认为提高了遮鱼的神经调节能力。(C)2003年爱思唯尔科学公司All rights reserved.
Juvenile milkfish Chanos chanos (Forssk (a) over circlel, 1775) were transferred from a local fish farm to fresh water (FW; 0parts per thousand), brackish water (BW, 10parts per thousand, 20parts per thousand) and seawater (SW; 35parts per thousand) conditions in the laboratory and reared for at least two weeks. The blood and gill of the fish adapted to various salinities were analyzed to determine the osmoregulatory ability of this euryhaline species. No significant difference was found in plasma osmolality, sodium or chloride concentrations of milkfish adapted to various salinities. In FW, the fish exhibited the highest specific activity of Na, K-ATPase (NKA) in gills, while the SW group was found to have the lowest. Relative abundance of branchial NKA alpha-subunit revealed similar profiles. However, in contrary to other euryhaline teleosts, i.e. tilapia, salmon and eel, the naturally SW-dwelling milkfish expresses higher activity of NKA in BW and FW. Immunocytochemical staining has shown that most Na, K-ATPase immunoreactive (NKIR) cells in fish adapted to BW and SW were localized to the filaments with very few on the lamellae. Moreover, in FW-adapted milkfish, the number of NKIR cells found on the lamellae increased significantly. Such responses as elevated NKIR cell number and NKA activity are thought to improve the osmoregulatory capacity of the milkfish in hyposaline environments. (C) 2003 Elsevier Science Inc. All rights reserved.