Tissue factor activity in whole blood
Tissue factor activity in whole blood
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DOI：
10.1182/blood-2004-09-3567
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发表时间：
2005-04-01
期刊：
影响因子：
20.3
通讯作者：
Mann, KG
中科院分区：
文献类型：
--
作者：
Butenas, S;Bouchard, BA;Mann, KG
组织因子（TF）是一种完整的膜蛋白，对止血至关重要。在过去的几年中，许多研究表明，生理活性TF在浓度大于30pm时可以作为血细胞和微粒的组成部分或作为可溶性血浆蛋白在血液中循环。在我们的研究中，使用含有活化血小板的接触途径抑制的血液或血浆，在没有外源性TF的情况下，通常20分钟内没有观察到凝块。在没有外源性TF的情况下，抑制性抗TF抗体对凝血时间也没有影响。在全血中添加浓度低至16至20 fM的TF会导致凝块形成的明显加速。使用离子载体处理的血小板，并采用功能和免疫分析来评估潜在血小板TF活性的存在。在静止或离子载体刺激的血小板上未观察到可检测到的TF活性或抗原。同样，在没有外源性TF的情况下，无TF抗抑制抗TF抗体对凝血时间也没有影响。在全血中添加低至16 ~ 20 fM浓度的TF会导致凝块形成的明显加速。使用离子载体处理的血小板，并采用功能和免疫分析来评估潜在血小板TF活性的存在。在静止或离子载体刺激的血小板上未观察到可检测到的TF活性或抗原。同样，在未刺激的全血中，单核细胞未检测到TF抗原，而在脂多糖（LPS）刺激的血液中，有相当一部分单核细胞表达TF。我们的数据表明，健康个体非细胞因子刺激血液中具有生理活性的TF浓度不能超过，可能低于20fm。
Tissue factor (TF) is an integral membrane protein essential for hemostasis. During the past several years, a number of studies have suggested that physiologically active TF circulates in blood at concentrations greater than 30 pM either as a component of blood cells and microparticles or as a soluble plasma protein. In our studies using contact pathway-inhibited blood or plasma containing activated platelets, typically no clot is observed for 20 minutes in the absence of exogenous TF. An inhibitory anti-TF antibody also has no effect on the clotting time in the absence of exogenous TF The addition of TF to whole blood at a concentration as low as 16 to 20 fM results in pronounced acceleration of clot formation. The presence of potential platelet TF activity was evaluated using ionophore-treated platelets and employing functional and immunoassays. No detectable TF activity or antigen was observed on quiescent or ionophore-stimulated platelets. Similarly, no TF antiinhibitory anti-TF antibody also has no effect on the clotting time in the absence of exogenous TF The addition of TF to whole blood at a concentration as low as 16 to 20 fM results in pronounced acceleration of clot formation. The presence of potential platelet TF activity was evaluated using ionophore-treated platelets and employing functional and immunoassays. No detectable TF activity or antigen was observed on quiescent or ionophore-stimulated platelets. Similarly, no TF antigen was detected on mononuclear cells in nonstimulated whole blood, whereas in lipopolysaccharicle (LPS)-stimulated blood a significant fraction of monocytes express TF. Our data indicate that the concentration of physiologically active TF in non-cytolkine-stimulated blood from healthy individuals cannot exceed and is probably lower than 20 fM.