NA AND K ACTIVITY COEFFICIENTS IN BILE AND BILE SALTS DETERMINED BY GLASS ELECTRODES.
NA AND K ACTIVITY COEFFICIENTS IN BILE AND BILE SALTS DETERMINED BY GLASS ELECTRODES.
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通过玻璃电极测定胆汁和胆汁盐中的 NA 和 K 活性系数。
DOI：
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发表时间：
1964
影响因子：
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通讯作者：
J. Dietschy
中科院分区：
文献类型：
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作者：
E. W. Moore;J. Dietschy
跨膜电位差的数学公式以离子活度而不是离子浓度来表示，并且需要了解混合溶液中给定离子种类的活度系数。阳离子选择性玻璃电极已用于测定纯胆汁盐溶液和天然胆汁中相对于标准 NaCl 或 KCl 溶液的钠和钾活度系数。与通过冰点降低测定的渗透系数进行比较。胆汁盐溶液和胆汁中的钠和钾活度系数均低于相应浓度的 NaCl 或 KCl 溶液，其中钾系数低于钠。这些导出的活性系数已在体内和体外胆囊制剂中进行实验使用，观察到的电位与霍奇金-卡茨方程预测的电位非常一致。
Mathematical formulations for transmembrane potential differences are expressed in terms of ionic activities rather than ionic concentrations, and require knowledge of the activity coefficients of a given ionic species in mixed solutions. Cation-selective glass electrodes have been used to determine sodium and potassium activity coefficients in pure bile salt solutions and in native bile, relative to standard NaCl or KCl solutions. Comparison was made with osmotic coefficients determined by freezing-point depression. Both sodium and potassium activity coefficients in bile salt solutions and in bile were lower than those for NaCl or KCl solutions at corresponding concentrations, with potassium coefficients being lower than those for sodium. These derived activity coefficients have been used experimentally in in vivo and in vitro gall bladder preparations with close agreement between observed potentials and those predicted by the Hodgkin-Katz equation.