Development of a Hybrid Artificial Liver using a Polyurethane Foam/Hepatocyte-Spheroid Packed-Bed Module
Development of a Hybrid Artificial Liver using a Polyurethane Foam/Hepatocyte-Spheroid Packed-Bed Module
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使用聚氨酯泡沫/肝细胞球体填充床模块开发混合人工肝
DOI：
10.1177/039139880002300607
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
K. Funatsu
中科院分区：
文献类型：
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作者：
H. Ijima;K. Nakazawa;S. Koyama;M. Kaneko;T. Matsushita;T. Gion;K. Shirabe;M. Shimada;K. Takenaka;K. Sugimachi;K. Funatsu
静态培养 1-2 天内，原代狗肝细胞在聚氨酯泡沫 (PUF) 的孔中自发形成球状体。球体直径约 100-150 μm，部分附着在表面并固定在这些孔内。利多卡因消失率在10天内降至约4μg/105个活细胞/天，而在PUF/球体培养物中，该速率几乎维持在8μg/105个活细胞/天的初始水平，持续10天。然后设计了两种规模的PUF填充床模块。小模块（PUF体积；14.5 cm3）用于体外培养以研究最佳培养条件，大模块（PUF体积；300 cm3）用于狗实验。接种在这些模块中的肝细胞也形成球体并保持几乎相同的白蛋白分泌率活性（小模块中为111μg/cm3 PUF/天，大模块中为87.7μg/cm3 PUF/天）。这些结果表明，含有肝细胞球体的 PUF 填充床模块有望作为混合人工肝
Primary dog hepatocytes spontaneously formed spheroids in the pores of polyurethane foam (PUF) within 1–2 days of stationary culture. The spheroids, about 100–150 μm in diameter, partly attached to the surface and immobilized inside these pores. The lidocaine disappearance rate decreased to about 4 μg/105 viable cells/day for 10 days, while in the PUF/spheroid culture the rate was maintained at almost the initial level of 8 μg/105 viable cells/day for 10 days. Then, two scales of PUF packed-bed modules were designed. A small module (PUF volume; 14.5 cm3) was used for in vitro culture to investigate optimum culture conditions, and a large module (PUF volume; 300 cm3) was designed for dog experiments. Hepatocytes inoculated in these modules also formed spheroids and maintained almost the same activity of albumin secretion rate (111 μg/cm3 PUF/day in the small module and 87.7 μg/cm3 PUF/day in the large module). These results indicate that the PUF packed-bed module containing hepatocyte-spheroids is promising as a hybrid artificial liver