Glutamine enhances glucose-induced mesangial cell proliferation
Glutamine enhances glucose-induced mesangial cell proliferation
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DOI：
10.1007/s00726-007-0002-9
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发表时间：
2008-05-01
期刊：
影响因子：
3.5
通讯作者：
Sellitti, Donald F.
中科院分区：
文献类型：
--
作者：
Lagranha, Claudia J.;Doi, Sonia Q.;Sellitti, Donald F.
分别在低糖(5 MM)和高糖(25 MM)培养液中测定谷氨酰胺(0-20 mM)对系膜细胞(MC)增殖的影响。高糖环境中的谷氨酰胺以剂量依赖的方式促进细胞增殖。抑制谷氨酰胺：果糖6-磷酸氨基转移酶(GFAT)和磷酸二酯酶可显著抑制谷氨酰胺诱导的细胞增殖。超生理水平的谷氨酰胺通过GFAT和cAMP依赖的途径增加HG环境中MC的增殖，提示谷氨酰胺可能对糖尿病肾病构成风险。
The proliferation of mesangial cells (MC) in the presence of glutamine (0-20 mM) was determined in both low (5 mM) and high (25 mM) glucose-containing medium. Glutamine in a high glucose (HG) environment increased cell proliferation in a dose-dependent manner. Inhibition of glutamine:fructose 6-phosphate amidotransferase (GFAT) and of phosphodiesterase significantly reduced glutamine-induced proliferation. Supraphysiologic levels of glutamine increase MC proliferation in a HG milieu via GFAT and cAMP-dependent pathways, suggesting that glutamine could pose a risk for diabetic nephropathy.