Tissue-specific laminin expression facilitates integrin-dependent association of the embryonic wing disc with the trachea in Drosophila
Tissue-specific laminin expression facilitates integrin-dependent association of the embryonic wing disc with the trachea in Drosophila
复制标题
DOI：
10.1016/j.ydbio.2006.12.022
复制
发表时间：
2007-04-01
影响因子：
2.7
通讯作者：
Hayashi, Shigeo
中科院分区：
文献类型：
--
作者：
Inoue, Yoshiko;Hayashi, Shigeo
异源组织的相互作用涉及由细胞外基质及其受体整合素介导的细胞粘附。果蝇翼盘是外胚层的内陷，接触基底外侧细胞表面的特定气管分支。我们发现，由翅泡（wb）编码的层粘连蛋白的α亚基对于建立翅和气管之间的相互作用至关重要。在胚胎发生过程中，翼盘细胞在其基底外侧表面呈现 Wb 并向后延伸，将它们的关联扩展到更靠后的气管分支。如果气管、Wb 或整合素缺失，这些迁移过程就会受到损害。延时和透射电子显微镜分析表明，Wb 促进大表面上的整合素依赖性接触，并控制翼细胞的细胞行为，包括其探索性丝状足活动。我们的数据确定 Wb 层粘连蛋白是一种细胞外基质配体，对于果蝇中整合素依赖性细胞迁移至关重要。 (c) 2006 Elsevier Inc. 保留所有权利。
The interaction of heterologous tissues involves cell adhesion mediated by the extracellular matrix and its receptor integrins. The Drosophila wing disc is an ectodermal invagination that contacts specific tracheal branches at the basolateral cell surface. We show that an alpha subunit of laminin, encoded by wing blister (wb), is essential for the establishment of the interaction between the wing and trachea. During embryogenesis, wing disc cells present Wb at their basolateral surface and extend posteriorly, expanding their association to more posteriorly located tracheal branches. These migratory processes are impaired in the absence of the trachea, Wb, or integrins. Time-lapse and transmission electron microscopy analyses suggest that Wb facilitates integrin-dependent contact over a large surface and controls the cellular behavior of the wing cells, including their exploratory filopodial activity. Our data identify Wb laminin as an extracellular matrix ligand that is essential for integrin-dependent cellular migration in Drosophila. (c) 2006 Elsevier Inc. All rights reserved.