Murine typhus in Mexico City
Murine typhus in Mexico City
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DOI：
10.1016/s0035-9203(00)90432-2
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发表时间：
2000-01-01
影响因子：
2.2
通讯作者：
Bravo-Lindoro, A
中科院分区：
文献类型：
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作者：
Acuna-Soto, R;Calderón-Romero, L;Bravo-Lindoro, A
为了确定斑疹伤寒在墨西哥城的流行情况，在墨西哥城国家儿科研究所血库的健康成人献血者中进行了血清学调查。研究了两组血清样品。第一组（n=100）用间接荧光抗体（IFA）法检测抗威尔明顿株伤寒立克次体总免疫球蛋白。第二组（n=1282）首先用Weil-Felix凝集试验筛选。如果滴度>1：80，则两种检测中的样本均被视为阳性。第一组IFA阳性14例（14%），第二组阳性193例（15.1%），男女均为Weil-Felix试验阳性。两种性别的阳性率差异有统计学意义（双尾χ2=3.33，95%CI =0.037- 0.14，P <0.05），其中49例（占女性总人数的20.1%）和144例（占男性总人数的13.9%）经Weil-Felix检验为阳性。从193份阳性血清中随机抽取90份进行IFA检测，其中88份为阳性。IFA检测后，性别频率的统计学差异得以维持。在对假阳性进行调整后，女性的比率估计为19.7%，男性为13.4%。抗R.在墨西哥城的健康成人中，伤寒杆菌感染率为16.7%。
To define the prevalence of murine typhus in Mexico City, a serosurvey was performed in a population of healthy adult blood donors from the blood bank of the National Institute of Paediatrics in Mexico City. Two groups of serum samples were studied. The first group (n=100) was tested by an indirect fluorescent antibody (IFA) method for the identification of total immunoglobulin against Wilmington strain ofRickettsia typhiby indirect immunofluorescence. The second group (n=1282) was first screened with the Weil-Felix agglutination test. Samples in both tests were considered positive if they had a titre >1:80. From the first group 14 (14%) were positive by IFA from the second group, 193 (15.1%) people of both sexes were positive to the Weil-Felix test. The rate of seropositivity was statistically different for the 2 sexes (2-tailed χ2=3.33, 95% CI=0.037-0.14,P<0.05) since 49 (20.1% of the total female population) and 144 (13.9% of the total male population) tested positive by Weil-Felix. From these 193 positive serum samples, 90 were randomly selected and tested with IFA, out of which 88 were confirmed positive. The statistical difference in the frequency by sex was maintained after IFA testing. After adjusting for false positives, the rate for women was estimated to be 19.7% and for men 13.4%. The prevalence of antibodies againstR. typhiin healthy adults of both sexes from Mexico City was 16.7%.