Investigation of the interaction between curcumin and nucleic acids in the presence of CTAB.
Investigation of the interaction between curcumin and nucleic acids in the presence of CTAB.
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DOI：
10.1016/j.saa.2006.07.027
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发表时间：
2007-06
期刊：
影响因子：
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通讯作者：
Feng Wang;Jinghe Yang;Xia Wu;Fei Wang;Honghong Ding
中科院分区：
文献类型：
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作者：
Feng Wang;Jinghe Yang;Xia Wu;Fei Wang;Honghong Ding
在阳离子表面活性剂十六烷基三甲基溴化铵（CTAB）存在下，核酸能增强姜黄素（CU）的共振光散射（RLS）增强效应。研究表明，在BR（pH 4.3）缓冲液中，阳离子CTAB和阴离子酵母RNA（yRNA）联合收割机结合形成阳离子大缔合物，并通过氢键和疏水力结合在CU羰基的两个碳原子上，形成CU-CTAB-yRNA三元复合物，导致该体系的共振光散射增强。在此基础上，建立了ngml-1处核酸的灵敏测定方法。
It is found that nucleic acid can enhance the resonance light scattering (RLS) enhancement effect of curcumin (CU) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB). The investigation indicates that in BR (pH 4.3) buffer, both the positive CTAB and negative yeast RNA (yRNA) combine and form a positive large association, then which is bound on the two carbon atoms of the carbonyls of CU through hydrogen bond and hydrophobic force and form CU–CTAB–yRNA ternary complex, resulting in the RLS enhancement of this system. Based on it, a sensitive method for determination of nucleic acids at ngml−1is established.