Cloning and expression profiling of the PacSnRK2 and PacPP2C gene families during fruit development, ABA treatment, and dehydration stress in sweet cherry
Cloning and expression profiling of the PacSnRK2 and PacPP2C gene families during fruit development, ABA treatment, and dehydration stress in sweet cherry
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甜樱桃果实发育、ABA 处理和脱水胁迫过程中 PacSnRK2 和 PacPP2C 基因家族的克隆和表达谱
DOI：
10.1016/j.plaphy.2017.08.025
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发表时间：
2017
影响因子：
6.5
通讯作者：
Li Tianhong
中科院分区：
文献类型：
--
作者：
Shen Xinjie;Guo Xiao;Zhao Di;Zhang Qiang;Jiang Yuzhuang;Wang Yantao;Peng Xiang;Wei Yan;Zhai Zefeng;Zhao Wei;Li Tianhong
植物SNF 1相关蛋白激酶2（SnRK 2）和蛋白磷酸酶2C（PP 2C）家族成员是阿坝信号转导途径的核心组成部分。SnRK 2和PP 2C蛋白被认为在果实成熟和提高植物对干旱胁迫的耐受性中起着关键作用，但支持甜樱桃（Prunus aviumL.）的遗传信息一直缺乏。本研究从甜樱桃品种中克隆了6个全长SnRK 2基因和3个全长PP 2C基因。红灯。定量PCR分析表明，外源阿坝处理对果实中PacSnRK 2. 2、PacSnRK 2. 3、PacSnRK 2. 6和PacPP 2C 1 - 3的表达有负调控作用，对PacSnRK 2. 4和PacSnRK 2. 5的表达有上调作用，而对PacSnRK 2. 1的表达无影响。阿坝处理还显著促进了甜樱桃果实中花青素的积累。干旱胁迫诱导了甜樱桃果实中所有PacSnRK 2和PacPP 2C基因的表达，并促进了阿坝、可溶性糖和花青素等干旱胁迫信号分子的积累。此外，酵母双杂交分析表明，PacPP 2C 1与所有六个PacSnRK 2相互作用，而PacPP 2C 3不与PacSnRK2.5相互作用。PacPP 2C 2不与PacSnRK2.1或PacSnRK2.4相互作用。这些结果表明，PacSnRK 2s和PacPP 2Cs在甜樱桃阿坝信号通路和果实对干旱胁迫的响应中可能发挥着多种作用。
Plant SNF1-related protein kinase 2 (SnRK2) and protein phosphatase 2C (PP2C) family members are core components of the ABA signal transduction pathway. SnRK2 and PP2C proteins have been suggested to play crucial roles in fruit ripening and improving plant tolerance to drought stress, but supporting genetic information has been lacking in sweet cherry (Prunus aviumL.). Here, we cloned six full-lengthSnRK2genes and three full-lengthPP2Cgenes from sweet cherry cv. Hong Deng. Quantitative PCR analysis revealed thatPacSnRK2.2,PacSnRK2.3,PacSnRK2.6, andPacPP2C1–3were negatively regulated in fruits in response to exogenous ABA treatment,PacSnRK2.4andPacSnRK2.5were upregulated, andPacSnRK2.1expression was not affected. The ABA treatment also significantly promoted the accumulation of anthocyanins in sweet cherry fruit. The expression of allPacSnRK2andPacPP2Cgenes was induced by dehydration stress, which also promoted the accumulation of drought stress signaling molecules in the sweet cherry fruits, including ABA, soluble sugars, and anthocyanin. Furthermore, a yeast two-hybrid analysis demonstrated that PacPP2C1 interacts with all six PacSnRK2s, while PacPP2C3 does not interact with PacSnRK2.5. PacPP2C2 does not interact with PacSnRK2.1 or PacSnRK2.4. These results indicate that PacSnRK2s and PacPP2Cs may play a variety of roles in the sweet cherry ABA signaling pathway and the fruit response to drought stress.