Determining the function of zebrafish epithalamic asymmetry
Determining the function of zebrafish epithalamic asymmetry
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DOI：
10.1098/rstb.2008.0234
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发表时间：
2009-04-12
影响因子：
6.3
通讯作者：
Halpern, Marnie E.
中科院分区：
文献类型：
--
作者：
Facchin, Lucilla;Burgess, Harold A.;Halpern, Marnie E.
与许多鱼类、两栖动物和爬行动物一样，斑马鱼（Danio rerio）的上丘脑具有明显的左右（L-R）不对称性。例如，在超过95%的斑马鱼幼鱼中，松果体的附属器官--松果体旁核形成于大脑的左侧，相邻的左侧缰核比右侧大。Nodal信号的中断影响这种偏差，产生相同数量的幼虫，其会阴旁位于左侧或右侧，以及相应的缰翻转。使用荧光转基因报告的活幼虫的预选择提供了一个有用的基板，研究神经解剖不对称行为的影响。先前的研究表明，上丘脑方向性与侧化行为（如L-R眼偏好）相关。我们发现，随机化的上丘脑不对称，通过干扰的大脑相关基因左撇子，不改变各种运动行为，包括反应偏侧刺激。然而，我们发现显着的赤字游泳启动和导航的总距离的幼虫与会阴反转。我们讨论了这些研究结果与以往的研究和最近的工作连接的缰区与控制的动机/奖励途径的脊椎动物的大脑。
As in many fishes, amphibians and reptiles, the epithalamus of the zebrafish, Danio rerio, develops with pronounced left-right (L-R) asymmetry. For example, in more than 95 per cent of zebrafish larvae, the parapineal, an accessory to the pineal organ, forms on the left side of the brain and the adjacent left habenular nucleus is larger than the right. Disruption of Nodal signalling affects this bias, producing equal numbers of larvae with the parapineal on the left or the right side and corresponding habenular reversals. Pre-selection of live larvae using fluorescent transgenic reporters provides a useful substrate for studying the effects of neuroanatomical asymmetry on behaviour. Previous studies had suggested that epithalamic directionality is correlated with lateralized behaviours such as L-R eye preference. We find that the randomization of epithalamic asymmetry, through perturbation of the nodal-related gene southpaw, does not alter a variety of motor behaviours, including responses to lateralized stimuli. However, we discovered significant deficits in swimming initiation and in the total distance navigated by larvae with parapineal reversals. We discuss these findings with respect to previous studies and recent work linking the habenular region with control of the motivation/ reward pathway of the vertebrate brain.