FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules.
FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules.
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DOI：
10.1080/15548627.2016.1261319
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发表时间：
2017-02
期刊：
影响因子：
13.3
通讯作者：
Kotaja N
中科院分区：
文献类型：
--
作者：
Da Ros M;Lehtiniemi T;Olotu O;Fischer D;Zhang FP;Vihinen H;Jokitalo E;Sironen A;Toppari J;Kotaja N
核糖核蛋白(RNP)颗粒在细胞质RNA调控中起着重要作用。具有异常多样化转录本的单倍体圆形精子细胞的特征是具有独特的生殖细胞特有的RNP颗粒，即类色素体(CB)。CB与体细胞RNP颗粒有许多相同的特征，但也具有种系特有的特征。CB似乎是PIWI相互作用RNA(PiRNA)靶向RNA调控的中心结构。在这里，我们确定了一个新的CB组分，FYCO1，它参与了体细胞自噬小泡的细胞内运输。我们证明了CB与自噬活性有关。通过对生殖细胞特异性Fyco1条件性基因敲除小鼠模型的分析，自噬诱导导致溶酶体小泡以FYCO1依赖的方式重新聚集到CB上。此外，在没有FYCO1的情况下，CB的完整性受到影响，CB被碎裂。我们的结果表明，RNP颗粒的动态平衡受到FYCO1介导的自噬的调节。
Ribonucleoprotein (RNP) granules play a major role in compartmentalizing cytoplasmic RNA regulation. Haploid round spermatids that have exceptionally diverse transcriptomes are characterized by a unique germ cell-specific RNP granule, the chromatoid body (CB). The CB shares many characteristics with somatic RNP granules but also has germline-specific features. The CB appears to be a central structure in PIWI-interacting RNA (piRNA)-targeted RNA regulation. Here, we identified a novel CB component, FYCO1, which is involved in the intracellular transport of autophagic vesicles in somatic cells. We demonstrated that the CB is associated with autophagic activity. Induction of autophagy leads to the recruitment of lysosomal vesicles onto the CB in a FYCO1-dependent manner as demonstrated by the analysis of a germ cell-specific Fyco1 conditional knockout mouse model. Furthermore, in the absence of FYCO1, the integrity of the CB was affected and the CB was fragmented. Our results suggest that RNP granule homeostasis is regulated by FYCO1-mediated autophagy.