ATP-REGULATED K+ CHANNELS IN CARDIAC-MUSCLE
ATP-REGULATED K+ CHANNELS IN CARDIAC-MUSCLE
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DOI：
10.1038/305147a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
NOMA, A
中科院分区：
文献类型：
--
作者：
NOMA, A
当心肌细胞被氰化物处理或缺氧时，未知性质的外向电流显著增加1 - 4，细胞内注射ATP5时，未知性质的外向电流减少。我们在这里报告了膜片钳技术在cn处理的哺乳动物心脏细胞中的应用，揭示了特定的K+通道，这些通道被细胞内ATP （ATPi）抑制的水平大于1mm。对于这些通道，向外的电导远大于向内的整流K+通道，而整流K+通道对ATP不敏感。AMP对ATP敏感的K+通道没有影响，ADP的作用不如ATP。因此，atp敏感的K+通道似乎在控制膜兴奋性的细胞能量代谢调节中起重要作用。
An outward current of unknown nature increases significantly when cardiac cells are treated with cyanide or subjected to hypoxia1–4, and decreases on intracellular injection of ATP5. We report here that application of the patch-clamp technique to CN-treated mammalian heart cells reveals specific K+channels which are depressed by intracellular ATP (ATPi) at levels greater than 1 mM. For these channels, conductance in the outward direction is much larger than the inward rectifier K+channel which is insensitive to ATP. AMP had no effect on the ATP-sensitive K+channel, and ADP was less effective than ATP. Thus, the ATP-sensitive K+channel seems to be important for regulation of cellular energy metabolism in the control of membrane excitability.