Hollow Nanoneedle Array and Its Utilization for Repeated Administration of Biomolecules to the Same Cells
Hollow Nanoneedle Array and Its Utilization for Repeated Administration of Biomolecules to the Same Cells
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DOI：
10.1021/nn300443h
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发表时间：
2012-06-01
期刊：
影响因子：
17.1
通讯作者：
Sivan, Uri
中科院分区：
文献类型：
--
作者：
Peer, Elad;Artzy-Schnirman, Arbel;Sivan, Uri
我们提出了一种新型的中空纳米针阵列（NNA）装置，能够在长时间内同时将不同的货物输送到培养物中的一组细胞中。二氧化硅针由一个共同的储液器供给，该储液器的内容物可以在真实的时间内补充和修改，同时保持与相同细胞的接触。NNA虽然具有亚微米的特性，但它是使用传统的大规模硅技术在绝缘体上硅晶片中制造的。显示3 T3-NIH成纤维细胞和HEK 293人胚肾细胞在NNA上成功生长和增殖。通过将荧光标记的葡聚糖重复给予相同的细胞并用编码红色荧光蛋白的DNA转染，证明了货物从储库通过针递送到培养物中的一组HEK 293细胞。NNA设备所展示的能力为大规模研究所选细胞对其环境的影响打开了大门，例如，在细胞命运决定的研究中，细胞自主与非自主机制在发育生物学中的作用，以及在可兴奋细胞网络的研究中。
We present a novel hollow nanoneedle array (NNA) device capable of simultaneously delivering diverse cargo into a group of cells in a culture over prolonged periods. The silica needles are fed by a common reservoir whose content can be replenished and modified in real time while maintaining contact with the same cells. The NNA, albeit its submicrometer features, is fabricated in a silicon-on-insulator wafer using conventional, large scale, silicon technology. 3T3-NIH fibroblast cells and HEK293 human embryonic kidney cells are shown to grow and proliferate successfully on the NNAs. Cargo delivery from the reservoir through the needles to a group of HEK293 cells in the culture is demonstrated by repeated administration of fluorescently labeled dextran to the same cells and transfection with DNA coding for red fluorescent protein. The capabilities demonstrated by the NNA device open the door to large scale studies of the effect of selected cells on their environment as encountered, for instance, in the study of cell-fate decisions, the role of cell-autonomous versus nonautonomous mechanisms in developmental biology, and in the study of excitable cell-networks.