Real-time observation of hemodynamic changes in glomerular aneurysms induced by anti-Thy-1 antibody.
Real-time observation of hemodynamic changes in glomerular aneurysms induced by anti-Thy-1 antibody.
复制标题
实时观察抗Thy-1抗体诱导的肾小球动脉瘤血流动力学变化。
作者：
Y. Oyanagi;J. Yao;Y. Wada;T. Morioka;Y. Suzuki;F. Gejyo;M. Arakawa;T. Oite
背景
微血管系统中的血流在确定炎症组织中损伤和修复的结果中起着关键作用。实时观察肾脏微血管，包括肾小球毛细血管丛，是非常困难的，因为目前可用的显微镜光学方法的限制。在本研究中，我们试图通过使用实时共聚焦激光扫描显微镜（CLSM）系统，从功能和定量上分析肾小球毛细血管簇破坏后微血管再生过程中体内发生的血流动力学事件。
方法
将聚乙烯导管插入颈动脉以测量血压。通过注射抗Thy-1.1抗体诱导产生微动脉瘤的系膜溶解病变。在抗体注射后第3天和第7天，我们在配备有实时CLSM的活体显微镜下结合高速CCD摄像机检查血流动力学变化。为了测量血管直径和红细胞速度，向大鼠注射异硫氰酸荧光素（FITC）标记的葡聚糖和FITC标记的红细胞（RBC）。
结果
在疾病的第3天，平均动脉血压为112 +/- 5 mm Hg，显著高于正常大鼠或第7天的大鼠（分别为93 +/- 1和101 +/- 9 mm Hg）。在第3天的微动脉瘤内，RBC速度被大大抑制。到第7天，在具有正常外观的肾小球中，RBC速度恢复到正常对照组中所见水平的一半左右（430.6 +/- 284.7 microm/sec），而在狭窄的肾小球簇中，RBC速度仍然只有104.6 +/- 35.1 microm/sec。
结论
非侵入性的程序，使用CLSM结合高速摄像机，使我们能够定量检查血流动力学事件，并分析微血管结构的三维肾脏。它可用于评价体内血流动力学反应和血管再生，并可能具有良好的临床应用前景。
BACKGROUND
Blood flow in the microvasculature plays a pivotal role in determining the outcome of injury and repair in inflamed tissue. Real-time observation of the kidney microvasculature, including the glomerular capillary tufts, is extremely difficult because of the methodological limitations of currently available microscope optics. In the present study, we attempted to analyze hemodynamic events that occurred in vivo during microvascular regeneration following destruction of the glomerular capillary tuft, functionally and quantitatively by the use of a real-time confocal laser-scanning microscope (CLSM) system.
METHODS
A polyethylene catheter was inserted into the carotid artery to allow blood pressure measurement. Mesangiolytic lesions producing microaneurysms were induced by the injection of anti-Thy-1.1 antibody. On days 3 and 7 after antibody injection, we examined hemodynamic changes under an intravital microscope equipped with real-time CLSM in combination with a high-speed CCD video camera. To measure vessel diameter and erythrocyte velocity, rats were injected with fluorescein isothiocyanate (FITC)-labeled dextran and FITC-labeled red blood cells (RBCs).
RESULTS
On day 3 of the disease, mean arterial blood pressure was 112 +/- 5 mm Hg, which was significantly higher than that of normal rat or of rats on day 7 (93 +/- 1 and 101 +/- 9 mm Hg, respectively). Within mircroaneurysms on day 3, RBC velocity was greatly suppressed. By day 7, RBC velocity, in glomeruli with normal appearances, recovered to about half of the level seen in normal controls (430.6 +/- 284.7 microm/sec), while in narrowed glomerular tufts, it was still only 104.6 +/- 35.1 microm/sec.
CONCLUSIONS
The noninvasive procedure, using CLSM in combination with a high-speed video camera, allowed us to examine hemodynamic events quantitatively and to analyze microvascular architecture three dimensionally in the kidney. It is useful for estimating hemodynamic response and vascular regeneration in vivo and may be promising for clinical application.
在哈布蛇毒引起的肾小球损伤中，系膜细胞迁移先于增殖。
DOI：
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发表时间：
1994
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
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作者：
Barnes,JL;Hevey,KA;Hastings,RR;Bocanegra,RA
通讯作者：
Bocanegra,RA