Morphogenetic movements underlying eye field formation require interactions between the FGF and ephrinB1 signaling pathways
Morphogenetic movements underlying eye field formation require interactions between the FGF and ephrinB1 signaling pathways
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DOI：
10.1016/s1534-5807(03)00395-2
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发表时间：
2004-01-01
影响因子：
11.8
通讯作者：
Moody, SA
中科院分区：
文献类型：
--
作者：
Moore, KB;Mood, K;Moody, SA
眼区的决定性视网膜祖细胞由转录因子指定，所述转录因子促进视网膜命运并控制对于眼区形成至关重要的细胞运动。然而，调节这些运动的分子信号通路在很大程度上是不确定的。我们证明了FGF和肝配蛋白通路都影响视野形成。在原肠胚形成之前激活FGF途径抑制假定的前神经板中的细胞运动，并防止细胞表达视网膜命运，独立于中胚层诱导或前后图案化。抑制FGF途径促进细胞分散并显著增加视野贡献。ephrinB1反向信号传导是促进细胞运动进入眼区所必需的，并且可以挽救FGF受体诱导的视网膜命运的抑制。这些结果表明，成纤维细胞生长因子调节肝配蛋白信号调节视网膜祖细胞的定位在最终的眼睛领域。
The definitive retinal progenitors of the eye field are specified by transcription factors that both promote a retinal fate and control cell movements that are critical for eye field formation. However, the molecular signaling pathways that regulate these movements are largely undefined. We demonstrate that both the FGF and ephrin pathways impact eye field formation. Activating the FGF pathway before gastrulation represses cellular movements in the presumptive anterior neural plate and prevents cells from expressing a retinal fate, independent of mesoderm induction or anterior-posterior patterning. Inhibiting the FGF pathway promotes cell dispersal and significantly increases eye field contribution. ephrinB1 reverse signaling is required to promote cellular movements into the eye field, and can rescue the FGF receptor-induced repression of retinal fate. These results indicate that FGF modulation of ephrin signaling regulates the positioning of retinal progenitor cells within the definitive eye field.