LIMITED RECEPTOR REPERTOIRE IN A MYCOBACTERIA-REACTIVE SUBSET OF GAMMA-DELTA-LYMPHOCYTES
LIMITED RECEPTOR REPERTOIRE IN A MYCOBACTERIA-REACTIVE SUBSET OF GAMMA-DELTA-LYMPHOCYTES
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DOI：
10.1038/342696a0
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发表时间：
1989-12-07
期刊：
影响因子：
64.8
通讯作者：
OBRIEN, RL
中科院分区：
文献类型：
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作者：
HAPP, MP;KUBO, RT;OBRIEN, RL
携带γδ T细胞受体（TCR）的淋巴细胞的生理作用尚不清楚。然而，这些细胞的一个子集的功能是从某些携带γδ链的淋巴细胞以受体依赖性方式被分枝杆菌抗原刺激的发现中推断出来的1 -5。我们发现，杂交瘤来源于这样的细胞在新生小鼠胸腺不仅响应分枝杆菌纯化蛋白衍生物（PPD），但也表现出明显的自身反应性1。在这两种反应是单倍型特异性的主要组织相容性（MHC）限制证明。为了研究这些γδ+细胞识别抗原的性质，我们对来自28个PPD反应性杂交瘤的γ-和δ-链进行了测序，发现需要一个特定的γ-链和一组有限的δ-链中的一个来产生PPD特异性。反应性γδ对表现出相当大的连接多样性，这可能会导致反应细胞的精细特异性差异。
THE physiological role of lymphocytes bearing theγδT-cell receptor (TCR) is still unclear. A function for a subset of these cells, however, is inferred from the finding that certainγδchain-bearing lymphocytes are stimulated in a receptor-dependent fashion by mycobacterial antigens1–5. We found that hybridomas derived from such cells in newborn murine thymus not only responded to mycobacterial purified protein derivative (PPD), but also exhibited an apparent autoreactivity1. In neither response was haplotype-specific major histocompatibility (MHC) restriction demonstrable. To investigate the nature of antigen recognition by theseγδ+cells, we sequenced theγ- andδ-chains from 28 PPD-reactive hybridomas, and found that a specificγ-chain, together with one of a limited set ofδ-chains, was needed to generate the PPD specificity. The reactiveγδpairs exhibited considerable junctional diversity, which may act to produce differences in the fine specificities of the responding cells.