Calbindin-28k-immunoreactivity in the trigeminal ganglion neurons and molar tooth pulp of the rat
Calbindin-28k-immunoreactivity in the trigeminal ganglion neurons and molar tooth pulp of the rat
复制标题
大鼠三叉神经节神经元和磨牙牙髓中 Calbindin-28k-免疫反应性
DOI：
10.1016/0006-8993(95)01550-7
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发表时间：
1996
期刊：
影响因子：
2.9
通讯作者：
T. Sugimoto
中科院分区：
文献类型：
--
作者：
H. Ichikawa;T. Deguchi;Y. Fujiyoshi;T. Nakago;D. Jacobowitz;T. Sugimoto
在大鼠三叉神经节（TG）中检查原代神经元的细胞体大小和碳酸酐酶（CA）、钙视网膜素（CR）和降钙素基因相关肽（CGRP）与钙结合蛋白-D28k（CB）的共表达。 CB 免疫反应性 (-ir) 细胞大多较大，并且优先分布在 TG 的上颌和下颌分区。 48% 的 CB-ir TG 细胞表现出酶 CA 活性。 10%的CB-ir TG细胞含有CR-ir。大多数共表达 CB- 和 CR-irs 的 TG 细胞定位于上颌和下颌分区并表现出 CA 活性。 6.5%的CB-ir TG细胞与CGRP-ir共存。 46% 共表达 CB 和 CGRP 的 TG 细胞表现出 CA 活性。还检查了 CB-ir TG 原代神经元对磨牙牙髓的神经支配。 CB-ir粗而光滑的神经纤维从根髓突出到牙髓角和牙髓室顶部，在那里它们变细并且很少进入牙本质细胞下层。然而，它们无法追溯到成牙本质细胞层、前牙本质或牙本质。 CB-ir 纸浆纤维的分布模式与 CR-ir 纸浆纤维的分布模式不同。用来自上颌磨牙牙髓的坚蓝（FB）逆行标记的三叉神经元细胞含有CB-和CR-irs。 23% 和 1% 的标记细胞分别对 CB 和 CR 产生免疫反应。 FB 标记细胞中 CB 和 CR 免疫反应性 (-irs) 的共表达可以忽略不计。免疫电镜方法显示，21%的牙髓神经纤维对CB有免疫反应，并且根髓中的所有CB-ir神经纤维都有髓鞘。本研究表明，牙髓初级神经元含有 CB-ir，但不共表达 CB-和 CR-ir，并且这些神经元将其有髓鞘轴突投射到牙髓。
The cell body size and coexpression of carbonic anhydrase (CA), calretinin (CR) and calcitonin gene-related peptide (CGRP) of primary neurons with calbindin-D28k (CB) was examined in the trigeminal ganglion (TG) of the rat. CB-immunoreactive (-ir) cells were mostly large and preferentially distributed in the maxillary and mandibular divisions of the TG. 48% of CB-ir TG cells exhibited enzyme CA activity. 10% of CB-ir TG cells contained CR-ir. Most TG cells coexpressing CB- and CR-irs were localized to the maxillary and mandibular divisions and exhibited CA activity. 6.5% of CB-ir TG cells coexisted with CGRP-ir. 46% of TG cells coexpressing CB and CGRP exhibited CA activity. The innervation of the molar tooth pulp by CB-ir TG primary neurons was also examined. CB-ir thick and smooth nerve fibers projected from the root pulp to the pulp horn and the roof of the pulp chamber, where they became thinner and rarely entered the subodontoblastic layer. However, they could not be traced to the odontoblastic layer, predentin or dentine. The distribution pattern of CB-ir pulpal fibers was different from that of CR-ir ones. The trigeminal neuronal cells retrogradely labeled with fast blue (FB) from the maxillary molar tooth pulp contained CB- and CR-irs. 23% and 1% of the labeled cells were immunoreactive for CB and CR, respectively. The coexpression of CB- and CR-immunoreactivities (-irs) in FB-labeled cells was negligible. An immunoelectron microscopic method revealed that 21% of pulpal nerve fibers were immunoreactive for CB, and that all CB-ir nerve fibers in the root pulp were myelinated. The present study indicated that the tooth pulp primary neurons contained CB-ir but did not coexpress CB-and CR-irs and that these neurons projected their myelinated axons to the pulp.
通过标记方法和大鼠神经毒素治疗研究细纤维皮肤神经支配及其表皮内贡献。
DOI：
10.3109/07367228509144573
发表时间：
1985
期刊：
Somatosensory research
影响因子：
--
作者：
Kruger,L;Sampogna,SL;Rodin,BE;Clague,J;Brecha,N;Yeh,Y
通讯作者：
Yeh,Y