Phospholipid Composition of Bacillus subtilis
Phospholipid Composition of Bacillus subtilis
复制标题
枯草芽孢杆菌的磷脂成分
DOI：
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发表时间：
1969
影响因子：
3.2
通讯作者：
L. M. Deenen
中科院分区：
文献类型：
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作者：
J. A. Kamp;I. Redai;L. M. Deenen
枯草芽孢杆菌含有至少五种磷脂，其中四种已被分离并鉴定为聚甘油磷脂，可能是心磷脂、磷脂酰甘油、磷脂酰乙醇胺和赖氨酰磷脂酰甘油。后者的磷酸甘油酯的进一步纯化，通过高压电泳获得，它表明，这种治疗去除含氨基酸，nonprophyldic材料从磷酸甘油酯。该相关材料（未与脂质共价连接）在赖氨酰磷脂酰甘油的酸水解产物中产生少量的除赖氨酸以外的多种氨基酸。B的磷脂组合物。枯草芽孢杆菌的生长似乎取决于生长条件。在培养基中加入葡萄糖降低了生长过程中的pH值，这是伴随着赖氨酰磷脂酰甘油的量的增加和磷脂酰甘油含量的减少，当与在中性pH值的生长相比。其他磷脂的量和磷脂的总量在不同的条件下保持恒定。该生物体的原生质体的形状和渗透敏感性似乎取决于生长条件。从中性生长培养基中收获的细胞得到球形原生质体，其迅速裂解，而在酸性培养基中生长的细胞在除去细胞壁后，甚至在悬浮于低渗培养基中后，在很大程度上保持其杆状形式。后一种观察结果表明，在生长期间暴露于低环境pH的细胞中存在更刚性的膜结构。
Bacillus subtilis contained at least five phospholipids, four of which have been isolated and identified as a polyglycerol phospholipid, probably cardiolipin, phosphatidylglycerol, phosphatidylethanolamine, and lysylphosphatidylglycerol. Further purification of the latter phosphoglyceride was obtained by high-voltage electrophoresis, and it was shown that this treatment removed amino acid-containing, nonlipidic material from the phosphoglyceride. This associated material, which is not covalently linked to the lipid, gave rise to minor amounts of a number of amino acids, other than lysine, in acid hydrolysates of the lysylphosphatidylglycerol. The phospholipid composition of B. subtilis appeared to depend on the growth conditions. Addition of glucose to the medium lowered the pH during growth; this was accompanied by an increase in the amount of lysylphosphatidylglycerol and a decrease in the phosphatidylglycerol content, when compared with growth at neutral pH. The amount of the other phospholipids and the total amount of phospholipid remained constant under the different conditions. The shape and the osmotic susceptibility of the protoplasts of this organism appeared to depend on the growth conditions. Cells harvested from a neutral growth medium gave spherical protoplasts which lysed rapidly, whereas cells grown in an acidic medium maintained their rod-shaped form to a great extent after the cell wall had been removed, even after being suspended in a hypotonic medium. The latter observation suggests the presence of a more rigid membranous structure in cells which have been exposed to a low environmental pH during growth.