The metabolic world of Escherichia coli is not small
The metabolic world of Escherichia coli is not small
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DOI：
10.1073/pnas.0306458101
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发表时间：
2004-02-10
影响因子：
11.1
通讯作者：
Arita, M
中科院分区：
文献类型：
--
作者：
Arita, M
为了阐明活生物体代谢的组织和进化原理，最近的研究已经解决了负责细胞构建块的合成和降解的大型生物化学网络的图论分析[Jeong，H.，Tombor，B.，艾伯特河，巴西-地奥尔特瓦伊角N. & Barabasi，A. L.（2000）Nature 407，651-654;瓦格纳，A. & Fell，D. A.（2001）Proc. R. Soc.伦敦系列B 268，1803-1810;和Ma，H.- W. & Zeng，A.- P.（2003）Bioinformatics 19，270-277]。在这些研究中，通过将酶反应视为代谢物之间的联系来计算网络的全局属性。然而，以这种方式计算的途径不保存其结构部分，因此不对应于传统代谢图上的生化途径。在这项工作中，我们重新评估了早期的结果，通过数字化大肠杆菌代谢反应中注释的碳原子痕迹。我们的分析表明，其代谢的平均路径长度比以前认为的要长得多，并且这种生物体的代谢世界在生物合成和降解方面并不小。
To elucidate the organizational and evolutionary principles of the metabolism of living organisms, recent studies have addressed the graph-theoretic analysis of large biochemical networks responsible for the synthesis and degradation of cellular building blocks [Jeong, H., Tombor, B., Albert, R., Oltvai, Z. N. & Barabasi, A. L. (2000) Nature 407, 651-654; Wagner, A. & Fell, D. A. (2001) Proc. R. Soc. London Ser. B 268, 1803-1810; and Ma, H.-W. & Zeng, A.-P. (2003) Bioinformatics 19, 270-277]. In such studies, the global properties of the network are computed by considering enzymatic reactions as links between metabolites. However, the pathways computed in this manner do not conserve their structural moieties and therefore do not correspond to biochemical pathways on the traditional metabolic map. in this work, we reassessed earlier results by digitizing carbon atomic traces in metabolic reactions annotated for Escherichia coli. Our analysis revealed that the average path length of its metabolism is much longer than previously thought and that the metabolic world of this organism is not small in terms of biosynthesis and degradation.