Isolation and characterization of endophytic bacteria from soybean (Glycine max) grown in soil treated with glyphosate herbicide
Isolation and characterization of endophytic bacteria from soybean (Glycine max) grown in soil treated with glyphosate herbicide
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DOI：
10.1007/s11104-004-6894-1
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发表时间：
2005-06-01
期刊：
影响因子：
4.9
通讯作者：
Azevedo, JL
中科院分区：
文献类型：
--
作者：
Kuklinsky-Sobral, HL;Araújo, WL;Azevedo, JL
内生细菌普遍存在于大多数植物物种中，通过抑制疾病、降解污染物和促进植物生长来影响宿主的适应性。这种内生细菌群落可能会受到作物管理的影响，例如化学化合物的使用。例如，草甘膦除草剂的应用很常见，主要是由于使用了抗草甘膦转基因植物。在这种情况下，植物与内生菌相互作用中的细菌平衡可能会发生变化，因为某些微生物群体能够使用草甘膦作为能量和营养物质的来源，而这种除草剂可能对其他群体有毒。因此，这项工作的目的是研究在施用和不施用草甘膦（种植前）的土壤中种植的大豆（Glycine max）植物的可培养和不可培养的内生细菌种群。从大豆叶、茎和根中回收的可培养内生细菌群落包括乙酸钙不动杆菌、朱氏不动杆菌、伯克霍尔德杆菌、唐菖蒲、坂崎肠杆菌、肺炎克雷伯菌、米生假单胞菌、麦草杆菌、皮氏拉尔斯顿菌和鞘氨醇单胞菌。对大豆根部进行的 DGGE（变性梯度凝胶电泳）分析揭示了一些未通过分离观察到的类群，这些类群是在种植前施用草甘膦的土壤中栽培的植物所特有的，例如 Herbaspirillum sp.，以及在没有草甘膦的土壤中栽培的植物中的其他类群，例如黄单胞菌属 sp.。和嗜麦芽寡养单胞菌。此外，通过草甘膦富集分离仅从大豆植物中回收了两种细菌。它们是米假单胞菌和唐菖蒲伯克霍尔德菌，它们对草甘膦表现出不同的敏感性。这些结果表明，种植前施用草甘膦除草剂可能会干扰内生细菌群落的平衡。该群落由不同物种组成，其促进植物生长和生物防治的能力可能受到影响。然而，这种处理在植物生产中的评价应通过在田间条件下的长期实验来进行。
Endophytic bacteria are ubiquitous in most plant species influencing the host fitness by disease suppression, contaminant degradation, and plant growth promotion. This endophytic bacterial community may be affected by crop management such as the use of chemical compounds. For instance, application of glyphosate herbicide is common mainly due to the use of glyphosate-resistant transgenic plants. In this case, the bacterial equilibrium in plant-endophyte interaction could be shifted because some microbial groups are able to use glyphosate as a source of energy and nutrients, whereas this herbicide may be toxic to other groups. Therefore, the aim of this work was to study cultivable and noncultivable endophytic bacterial populations from soybean (Glycine max) plants cultivated in soil with and without glyphosate application (preplanting). The cultivable endophytic bacterial community recovered from soybean leaves, steins, and roots included Acinetobacter calcoaceticus, A. junii, Burkholderia sp., B. gladioli, Enterobacter sakazaki, Klebsiella pneumoniae, Pseudomonas oryzihabitans, P. straminea, Ralstonia pickettii, and Sphingoimonas sp. The DGGE (Denaturing Gradient Gel Electrophoresis) analysis from soybean roots revealed some groups not observed by isolation that were exclusive for plants cultivated in soil with pre-planting glyphosate application, such as Herbaspirillum sp., and other groups in plants that were cultivated in soil without glyphosate, such as Xanthomonas sp. and Stenotrophomonas maltophilia. Furthermore, only two bacterial species were recovered from soybean plants by glyphosate enrichment isolation. They were Pseudomonas oryzihabitans and Burkholderia gladioli which showed different sensibility profiles to the glyphosate. These results suggest that the application at pre-planting of the glyphosate herbicide may interfere with the endophytic bacterial community's equilibrium. This community is composed of different species with the capacity for plant growth promotion and biological control that may be affected. However, the evaluation of this treatment in plant production should be carried out by long-term experiments in field conditions.