COEXPRESSION OF ALPHA-SARCOMERIC ACTIN, ALPHA-SMOOTH MUSCLE ACTIN AND DESMIN DURING MYOGENESIS IN RAT AND MOUSE EMBRYOS .1. SKELETAL-MUSCLE
COEXPRESSION OF ALPHA-SARCOMERIC ACTIN, ALPHA-SMOOTH MUSCLE ACTIN AND DESMIN DURING MYOGENESIS IN RAT AND MOUSE EMBRYOS .1. SKELETAL-MUSCLE
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DOI：
10.1111/j.1432-0436.1990.tb00546.x
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发表时间：
1990-08-01
期刊：
影响因子：
2.9
通讯作者：
GABBIANI, G
中科院分区：
文献类型：
--
作者：
BABAI, F;MUSEVIAGHDAM, J;GABBIANI, G
波形蛋白、结蛋白、α-肌节和α-用免疫组织化学方法检测了大鼠和小鼠胚胎组织中平滑肌肌动蛋白。结果显示结蛋白和α-谷氨酰胺的表达相似。肌动蛋白同种型（α- Sr和α- sm）在小鼠胎儿-胚胎发育期间骨骼肌细胞中的表达。在这两个物种中，α- Sr和α-在成心肌细胞、肌瘤成肌细胞和肌管中观察到SM肌动蛋白。α的强度SM肌动蛋白的表达在肌发生的终末阶段减少，在成熟的心肌细胞和肌纤维中完全消失。结蛋白在所有融合前成肌细胞（1型和2型成肌细胞）、肌管和肌纤维中表达。在体节的成肌细胞中结蛋白的出现之前几小时，肌动蛋白（α- Sr和α- sm）。我们对波形蛋白表达的研究仅限于大鼠胚胎，揭示了体节前成肌细胞仅表达波形蛋白，1型成肌细胞表达波形蛋白和结蛋白，2型成肌细胞（横纹肌细胞）表达结蛋白和α-肌动蛋白（α- Sr和α- sm）。我们的研究证明了胎儿-胚胎肌发生和肌源性肿瘤分化之间的相似性：结蛋白出现在α-肌动蛋白之前。肌动蛋白在胚胎成肌细胞中的表达，并且可以被认为是肌源性分化的初始步骤的标志物。α- SM肌动蛋白被认为是横纹肌细胞胎胚肌动蛋白，在发育过程中在骨骼肌成肌细胞和成心肌细胞中瞬时表达，并在骨骼肌的肿瘤转化过程中重新出现。
Expression of vimentin, desmin, .alpha.-sarcomeric and .alpha.-smooth muscle actins in embryonic tissues of rat and mice was examined using an immunohistochemical approach. The results showed a similarity in the expression of desmin and .alpha.-actin isoforms (.alpha.-sr and .alpha.-sm) in skeletal muscle cells during murine feto-embryonic development. In the two species, coexpression of .alpha.-sr and .alpha.-sm actins has been observed in cardiomyoblasts, myotomal myoblasts and myotubes. The intensity of .alpha.-sm actin expression decreased during the terminal steps of myogenesis and disappeared completely in mature cardiomyocytes and myofibres. Desmin was expressed in all prefusion myoblasts (type 1 and 2 myoblasts), myotubes, and in myofibres. The appearance of desmin in myoblasts of somites preceded by a few hours the expression of the .alpha.-actins (.alpha.-sr and .alpha.-sm). Our study on vimentin expression, limited to rat embryos, revealed that somite premyoblasts expressed only vimentin, type 1 myoblasts expressed vimentin and desmin, and type 2 myoblasts (rhabdomyoblasts) expressed desmin and .alpha.-actins (.alpha.-sr and .alpha.-sm). Our study demonstrates the resemblance between feto-embryonic myogenesis and myogenic neoplastic differentiation: desmin appears before the .alpha.-actins in embryonic myoblasts, and can be considered as a marker of an initial step in myogenic differentiation. .alpha.-sm actin, considered as a striated muscle cell feto-embryonic actin, is expressed transiently in skeletal myoblasts and cardiomyoblasts during development and reappears during neoplastic transformation of skeletal muscle.