Increase of essential amino acids in the bovine uterine lumen during preimplantation development
Increase of essential amino acids in the bovine uterine lumen during preimplantation development
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DOI：
10.1530/rep-10-0533
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发表时间：
2011-05-01
期刊：
影响因子：
3.8
通讯作者：
Ulbrich, Susanne E.
中科院分区：
文献类型：
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作者：
Groebner, Anna E.;Rubio-Aliaga, Isabel;Ulbrich, Susanne E.
氨基酸（AAs）对着床前发育中的孕体至关重要。为了深入了解牛胚胎的要求，我们确定了子宫液的AA组成。在发情后第12、15和18天，对同步妊娠和非妊娠西门塔尔小母牛的子宫进行冲洗，以通过液相色谱-串联质谱法分析41种AA及其衍生物。取同侧子宫内膜进行定量PCR。除了妊娠依赖性的必需氨基酸增加外（P
Amino acids (AAs) are crucial for the developing conceptus prior to implantation. To provide insights into the requirements of the bovine embryo, we determined the AA composition of the uterine fluid. At days 12, 15, and 18 post-estrus, the uteri of synchronized pregnant and non-pregnant Simmental heifers were flushed for the analysis of 41 AAs and their derivatives by liquid chromatography-tandem mass spectrometry. The ipsilateral endometrium was sampled for quantitative PCR. In addition to a pregnancy-dependent increase of the essential AAs (P