Generalized Fatal Cowpox virus Infection in a Cat with Transmission to a Human Contact Case
Generalized Fatal Cowpox virus Infection in a Cat with Transmission to a Human Contact Case
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猫全身性致命牛痘病毒感染并传播给人类的病例
作者：
Christian Schulze;M. Alex;H. Schirrmeier;A. Hlinak;A. Engelhardt;B. Koschinski;B. Beyreiß;M. Hoffmann;Claus
一只4个月大的雌性家养短毛猫感染了痘病毒科病毒。这只动物出现了严重的肺炎和全身皮肤溃疡。组织学上，存在典型的嗜酸性细胞浆内包涵体，表明正痘病毒（OPV）感染。肺呈灰白色至出血性结节状病变，中心区肺泡和支气管组织完全坏死。皮肤和肺结节电镜显示典型的方形OPV颗粒。在鸡胚绒毛膜-尿囊膜上培养病毒可导致出血性斑块。限制性内切酶分析、PCR和D8L基因测序鉴定该分离株为典型的牛痘病毒。该病毒由猫传播给人类接触者，该接触者在接种部位出现局部结节性皮炎，伴有全身感染迹象，配对血清样本中OPV特异性中和抗体增加。
A 4‐month‐old female domestic shorthair cat was infected by a virus of the Poxvirus family. The animal developed a severe pneumonia and generalized ulcerating lesions of the skin. Histologically, typical eosinophilic intracytoplasmic inclusion bodies indicative of an Orthopoxvirus (OPV) infection were present. The lung showed grey‐white to haemorrhagic nodular lesions with a central zone of complete necrosis of alveolar and bronchial tissue. Electron microscopy from skin and lung nodules revealed typical square‐shaped OPV particles. Cultivation of the virus on chorio‐allantoic membranes of embryonated chicken eggs resulted in haemorrhagic plaques. Restriction enzyme analysis, PCR and sequencing of the D8L gene identified the OPV isolate as a typical Cowpox virus. It was transmitted by the cat to a human contact person who developed a local nodular dermatitis at the inoculation site in association with signs of general infection and had an increase of OPV‐specific neutralizing antibodies in paired serum samples.